MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change.
| Main Author: | |
|---|---|
| Format: | Dataset |
| Language: | English |
| Published: |
MassIVE
2020
|
| Subjects: | |
| Online Access: | https://dx.doi.org/10.25345/c5546q https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000085868 |
| Description not available. |