MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).

Bibliographic Details
Main Author: Khudyakov, Jane
Format: Dataset
Language:English
Published: MassIVE 2021
Subjects:
Elephant Seal
Online Access:https://dx.doi.org/10.25345/c5225q
https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000088122
id ftdatacite:10.25345/c5225q
record_format openpolar
spelling ftdatacite:10.25345/c5225q 2023-05-15T16:05:08+02:00 MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio). Khudyakov, Jane 2021 https://dx.doi.org/10.25345/c5225q https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000088122 en eng MassIVE Mass Spectrometry dataset Dataset 2021 ftdatacite https://doi.org/10.25345/c5225q 2021-11-05T12:55:41Z Dataset Elephant Seal DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language English
format Dataset
author Khudyakov, Jane
spellingShingle Khudyakov, Jane
MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
author_facet Khudyakov, Jane
author_sort Khudyakov, Jane
title MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
title_short MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
title_full MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
title_fullStr MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
title_full_unstemmed MassIVE MSV000088122 - Northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : Northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
title_sort massive msv000088122 - northern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting : northern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see sample key in supplementary files). proteins were isolated from muscle using the trizol method and blubber using qiazol with the qiagen rneasy lipid mini kit (see khudyakov et al., 2018, doi: 10.1242/bio.036731). plasma samples were used raw (without depletion of highly abundant proteins). muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 m urea, and 5 mm dtt in 50 mm ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
publisher MassIVE
publishDate 2021
url https://dx.doi.org/10.25345/c5225q
https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000088122
genre Elephant Seal
genre_facet Elephant Seal
op_doi https://doi.org/10.25345/c5225q
_version_ 1766400926390681600

Similar Items