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Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm,...
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Online Access: | https://dx.doi.org/10.22002/wwye5-1ba13 https://data.caltech.edu/doi/10.22002/wwye5-1ba13 |
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ftdatacite:10.22002/wwye5-1ba13 2023-07-23T04:18:13+02:00 lip7-3 (ctrl) ... Nicolas, William 2020 mp4 jpg mrc rec https://dx.doi.org/10.22002/wwye5-1ba13 https://data.caltech.edu/doi/10.22002/wwye5-1ba13 en eng CaltechDATA Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode cc-by-nc-4.0 Escherichia coli PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor dataset Dataset 2020 ftdatacite https://doi.org/10.22002/wwye5-1ba13 2023-07-03T17:30:15Z Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and further purification by . ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing through Potter and sonicator.. Sample Preparation: Fiducials were prepared my method (see lab archive). ... Dataset artica DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
topic |
Escherichia coli PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor |
spellingShingle |
Escherichia coli PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor Nicolas, William lip7-3 (ctrl) ... |
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Escherichia coli PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Single Axis IECB Talos Artica Serial EM pipeline Raptor |
description |
Exposure 1.5s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 56.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: . . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and further purification by . ultracentrifugation of the membrane fractions. . Liposomes are then generated from the membrane fractions by processing through Potter and sonicator.. Sample Preparation: Fiducials were prepared my method (see lab archive). ... |
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Dataset |
author |
Nicolas, William |
author_facet |
Nicolas, William |
author_sort |
Nicolas, William |
title |
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publisher |
CaltechDATA |
publishDate |
2020 |
url |
https://dx.doi.org/10.22002/wwye5-1ba13 https://data.caltech.edu/doi/10.22002/wwye5-1ba13 |
genre |
artica |
genre_facet |
artica |
op_rights |
Creative Commons Attribution Non Commercial 4.0 International https://creativecommons.org/licenses/by-nc/4.0/legalcode cc-by-nc-4.0 |
op_doi |
https://doi.org/10.22002/wwye5-1ba13 |
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1772180425482960896 |