lip9-3 (+ megacomplex) ...

• Main Author: Nicolas, William
• Format: Dataset
• Language: English
• Published: CaltechDATA 2020
• Subjects: Escherichia coli; PK2, BL21 dHisdHA RQABpCDF+EFGpRSF; Single Axis; IECB Talos Artica; Serial EM; pipeline; Raptor; artica
• Online Access: https://dx.doi.org/10.22002/n7ne9-cdk22; https://data.caltech.edu/doi/10.22002/n7ne9-cdk22

Exposure 1s ... : Tilt Series Date: 2020-10-05 Data Taken By: William Nicolas Species / Specimen: Escherichia coli Strain: PK2, BL21 dHisdHA RQABpCDF+EFGpRSF Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 88.0 eV/Ų, defocus: -10.0 μm, magnification: 11000x. Microscope: IECB Talos Artica Acquisition Software: Serial EM Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Petya Krasteva (head of the SBB lab at the IECB, Bordeaux° and Wiem . Abidi (graduate student) did the preps and helped with acquisition Purification / Growth Conditions / Treatment: BL21 cells were transformed with QAB - pCDF plasmid and EFG - pRSF . plasmid to express the inner-membrane macro-complex.. . Selection done with Kanamycin and Streptomycin.. . Induction of expression ON done with IPTG. . Spheroplasts are generated from the BL21 non transformed cells cells.. Spheroplasts are processed through a high-pressure homogenizer and . further purification by ...