Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae)
This study demonstrated the presence of lipofuscin granules in the olfactory lobe cell mass (OLCM) of snow crab Chionoecetes opilio and examined whether granule quantification results are subject to methodological bias. Lipofuscin quantification in histological sections of the OLCM were compared bet...
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ftdatacite:10.21411/cbm.a.352973f6 2023-05-15T15:54:08+02:00 Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) ALLAIN, Renée MORIYASU, Mikio CRAWFORD, Bryan COURTENAY, Simon 2011 https://dx.doi.org/10.21411/cbm.a.352973f6 http://application.sb-roscoff.fr/cbm/doi/10.21411/CBM.A.352973F6 unknown Station Biologique de Roscoff (SBR) https://dx.doi.org/10.21411/cbm.i.f1a94f34 Text Article article-journal ScholarlyArticle 2011 ftdatacite https://doi.org/10.21411/cbm.a.352973f6 https://doi.org/10.21411/cbm.i.f1a94f34 2021-11-05T12:55:41Z This study demonstrated the presence of lipofuscin granules in the olfactory lobe cell mass (OLCM) of snow crab Chionoecetes opilio and examined whether granule quantification results are subject to methodological bias. Lipofuscin quantification in histological sections of the OLCM were compared between: 1) snow crab categories (sex and two indicators of age: size and condition of carapace (evaluated by the coloration of the carapace, presence/absence of scars and epizootic and epiphytic growth); 2) sample preparation methods; and 3) microscopic image collection parameters. No significant difference was observed in lipofuscin levels between mature males and females of similar size and carapace condition. Lipofuscin was more abundant in crabs with a decalcifying (presumably, older) carapace condition. However, larger clean-shell males did not have higher lipofuscin levels than smaller old-decalcifying-shell males. Autofluorescent intensity of granules was significantly higher in brain samples post-fixed in decalcifying solution compared to formalin fixed samples; however, lipofuscin levels did not differ. Increased exposure times to laser light of up to 120 seconds significantly decreased autofluorescent intensity of lipofuscin granules. No significant differences were observed in lipofuscin levels when varying optical section thickness or when comparing single confocal planes and stacks. Lipofuscin granule size frequency histograms showed possible image analysis limitations in terms of minimum lipofuscin granule size. These results raised concerns regarding the applicability of histological lipofuscin quantification in the OLCM of snow crabs. Text Chionoecetes opilio Snow crab DataCite Metadata Store (German National Library of Science and Technology) |
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This study demonstrated the presence of lipofuscin granules in the olfactory lobe cell mass (OLCM) of snow crab Chionoecetes opilio and examined whether granule quantification results are subject to methodological bias. Lipofuscin quantification in histological sections of the OLCM were compared between: 1) snow crab categories (sex and two indicators of age: size and condition of carapace (evaluated by the coloration of the carapace, presence/absence of scars and epizootic and epiphytic growth); 2) sample preparation methods; and 3) microscopic image collection parameters. No significant difference was observed in lipofuscin levels between mature males and females of similar size and carapace condition. Lipofuscin was more abundant in crabs with a decalcifying (presumably, older) carapace condition. However, larger clean-shell males did not have higher lipofuscin levels than smaller old-decalcifying-shell males. Autofluorescent intensity of granules was significantly higher in brain samples post-fixed in decalcifying solution compared to formalin fixed samples; however, lipofuscin levels did not differ. Increased exposure times to laser light of up to 120 seconds significantly decreased autofluorescent intensity of lipofuscin granules. No significant differences were observed in lipofuscin levels when varying optical section thickness or when comparing single confocal planes and stacks. Lipofuscin granule size frequency histograms showed possible image analysis limitations in terms of minimum lipofuscin granule size. These results raised concerns regarding the applicability of histological lipofuscin quantification in the OLCM of snow crabs. |
format |
Text |
author |
ALLAIN, Renée MORIYASU, Mikio CRAWFORD, Bryan COURTENAY, Simon |
spellingShingle |
ALLAIN, Renée MORIYASU, Mikio CRAWFORD, Bryan COURTENAY, Simon Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
author_facet |
ALLAIN, Renée MORIYASU, Mikio CRAWFORD, Bryan COURTENAY, Simon |
author_sort |
ALLAIN, Renée |
title |
Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
title_short |
Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
title_full |
Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
title_fullStr |
Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
title_full_unstemmed |
Technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs Chionoecetes opilio (Decapoda: Oregoniidae) |
title_sort |
technical development and calibration of lipofuscin quantification for the potential use as an age estimate in snow crabs chionoecetes opilio (decapoda: oregoniidae) |
publisher |
Station Biologique de Roscoff (SBR) |
publishDate |
2011 |
url |
https://dx.doi.org/10.21411/cbm.a.352973f6 http://application.sb-roscoff.fr/cbm/doi/10.21411/CBM.A.352973F6 |
genre |
Chionoecetes opilio Snow crab |
genre_facet |
Chionoecetes opilio Snow crab |
op_relation |
https://dx.doi.org/10.21411/cbm.i.f1a94f34 |
op_doi |
https://doi.org/10.21411/cbm.a.352973f6 https://doi.org/10.21411/cbm.i.f1a94f34 |
_version_ |
1766389314185330688 |