Quantity and quality of isolated RNA and transcript levels of selected genes in killer whale fibroblasts exposed to pollutants ...

• Main Authors: Routti, Heli, Bjørneset, Juni, Dalmo, Roy Ambli, Goksøyr, Anders, Limonta, Giacomo, Panti, Cristina, Rikardsen, Audun, Harju, Mikael, Yadetie, Fekadu, Blévin, Pierre
• Format: Dataset
• Language: unknown
• Published: npolar.no 2022
• Subjects: Norway; Killer Whale; Northern Norway; Killer whale; Lyngen
• Online Access: https://dx.doi.org/10.21334/npolar.2022.a6d075bb; https://data.npolar.no/dataset/a6d075bb-69ad-4dbc-b59e-f1b0f9b3e8a3

The dataset shows quantity and quality of isolated RNA and transcript levels of selected genes in killer whale fibroblasts exposed to pollutants. A fibroblast culture was established from a killer whale skin/blubber biopsy collected in November 2020 from Lyngen, Norway. The cells of passage 3-4 were exposed to a mixture of pollutants over 48 hours. The applied pollutant concentrations were 0.1X, 1X and 10X (column measurementID) i.e., 1.16 µM, 11.57 µM and 115.7 µM, respectively, where 1X reflected median levels of the ten most abundant persistent organic pollutants in killer whale blubber from Northern Norway (p,p’-DDE > PCB-153 > PCB-138 > trans-nonachlor > PCB-180 > PCB-101 = PCB-52 > PCB99 > PCB187 > PCB-118). The selected genes include aryl hydrocarbon receptor (AHR), cytochrome P450 1A (CYP1A), cytochrome P450 3A (CYP3A), cytochrome P450 4A (CYP4A), thyroid hormone receptor alpha (THRA), thyroid hormone receptor beta (THRB), glucocorticoid receptor (GR), peroxisome proliferator ...