Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ...
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtain...
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Online Access: | https://dx.doi.org/10.21256/zhaw-26000 https://digitalcollection.zhaw.ch/handle/11475/26000 |
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ftdatacite:10.21256/zhaw-26000 2023-07-23T04:15:43+02:00 Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco 2020 application/pdf https://dx.doi.org/10.21256/zhaw-26000 https://digitalcollection.zhaw.ch/handle/11475/26000 en eng MDPI Antarctica Chionodraco hamatus Cold adaptation Gene expression Molecular evolution Protein purification Superoxide dismutase Teleost 572 Biochemie 590 Tiere Zoologie Journal Article article-journal ScholarlyArticle Text 2020 ftdatacite https://doi.org/10.21256/zhaw-26000 2023-07-03T22:13:13Z In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the "unclock-like" behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the ... Text Antarc* Antarctic Antarctica antartic* Icefish Ross Sea DataCite Metadata Store (German National Library of Science and Technology) Antarctic Ross Sea The Antarctic |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Antarctica Chionodraco hamatus Cold adaptation Gene expression Molecular evolution Protein purification Superoxide dismutase Teleost 572 Biochemie 590 Tiere Zoologie |
spellingShingle |
Antarctica Chionodraco hamatus Cold adaptation Gene expression Molecular evolution Protein purification Superoxide dismutase Teleost 572 Biochemie 590 Tiere Zoologie Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
topic_facet |
Antarctica Chionodraco hamatus Cold adaptation Gene expression Molecular evolution Protein purification Superoxide dismutase Teleost 572 Biochemie 590 Tiere Zoologie |
description |
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the "unclock-like" behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the ... |
format |
Text |
author |
Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco |
author_facet |
Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco |
author_sort |
Chatzidimitriou, Evangelia |
title |
Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
title_short |
Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
title_full |
Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
title_fullStr |
Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
title_full_unstemmed |
Copper/Zinc superoxide dismutase from the crocodile icefish Chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
title_sort |
copper/zinc superoxide dismutase from the crocodile icefish chionodraco hamatus : antioxidant defense at constant sub-zero temperature ... |
publisher |
MDPI |
publishDate |
2020 |
url |
https://dx.doi.org/10.21256/zhaw-26000 https://digitalcollection.zhaw.ch/handle/11475/26000 |
geographic |
Antarctic Ross Sea The Antarctic |
geographic_facet |
Antarctic Ross Sea The Antarctic |
genre |
Antarc* Antarctic Antarctica antartic* Icefish Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica antartic* Icefish Ross Sea |
op_doi |
https://doi.org/10.21256/zhaw-26000 |
_version_ |
1772176683686690816 |