Methyl-coenzyme M reductase alpha subunit (mcrA) transcripts from Arctic tundra soil near Utqiagvik, Alaska, 2018

• Main Author: Lipson, David
• Format: Dataset
• Language: English
• Published: NSF Arctic Data Center 2021
• Subjects: Arctic tundra; mcrA; methanogen; microbial community; Arctic; permafrost; Tundra; Alaska
• Online Access: https://dx.doi.org/10.18739/a2bk16q88; https://arcticdata.io/catalog/view/doi:10.18739/A2BK16Q88

When wet Arctic tundra soils begin to freeze in the fall, an unfrozen layer remains between the frozen surface and deeper permafrost layers. This period is known as the zero curtain, as liquid water keeps the temperature of this soil layer near 0 Celsius (C) while latent heat is gradually dissipated. This project investigates the methanogenic Archaea that are metabolically active in the unfrozen layer during the fall zero curtain period and compares this community to that which is active in the late summer at the same depth (10-20 centimeters (cm)). This dataset contains the abundance of distinct partial mcrA (Methyl-coenzyme M reductase alpha subunit) gene sequences (operational taxonomic units, OTU's defined at 16% similarity) amplified from messenger ribonucleic acid (mRNA) extracted from soil samples in this study. These data complement the sequences deposited in GenBank (accession numbers OL505703-OL505708).