The relevance of molecular biomarkers as a component of a weight-of-evicence aaproach to marine monitoring ...

• Main Authors: Bean, T. P., Bignell, J. P., Thain, J. E., Lyons, B.P.
• Format: Conference Object
• Language: unknown
• Published: ASC 2010 - Theme session F 2024
• Subjects: Ecosystem observation, processes and dynamics; Iceland
• Online Access: https://dx.doi.org/10.17895/ices.pub.25069208; https://ices-library.figshare.com/articles/conference_contribution/The_relevance_of_molecular_biomarkers_as_a_component_of_a_weight-of-evicence_aaproach_to_marine_monitoring/25069208

No abstracts are to be cited without prior reference to the author.Integrated monitoring programmes measuring the biological effects of contaminants in our seas are now fully incorporated into national and international monitoring activities; with the key aim of identifying and understanding anthropogenic changes in ecosystem health. As part of these monitoring activities we have sampled two well characterized indicator species from sites around the UK and compared these directly with pristine sites in Iceland. Samples were analysed with a suite of traditional biomarkers (measurements included condition index, comet assay, stress, histopathology, and enzyme-based assays) and assessed for the presence of chemical contaminants. Alongside these traditional biomarker techniques we also used real-time PCR to assess gene expression, which has previously been associated with increased levels of contaminants. Genes studied in flounder include CYP1A, vitellogenin, metallothionein, and thyroid receptors, expression of ...