Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data
We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed u...
Main Author: | |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
Mendeley
2021
|
Subjects: | |
Online Access: | https://dx.doi.org/10.17632/5mkvk5yc4w.2 https://data.mendeley.com/datasets/5mkvk5yc4w/2 |
id |
ftdatacite:10.17632/5mkvk5yc4w.2 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.17632/5mkvk5yc4w.2 2023-05-15T17:58:44+02:00 Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data Menzorov, Aleksei 2021 https://dx.doi.org/10.17632/5mkvk5yc4w.2 https://data.mendeley.com/datasets/5mkvk5yc4w/2 unknown Mendeley https://dx.doi.org/10.17632/5mkvk5yc4w Creative Commons Attribution 4.0 International info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Stem Cell Transcriptome Seal dataset Dataset 2021 ftdatacite https://doi.org/10.17632/5mkvk5yc4w.2 https://doi.org/10.17632/5mkvk5yc4w 2021-11-05T12:55:41Z We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis. Dataset Phoca hispida ringed seal DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Stem Cell Transcriptome Seal |
spellingShingle |
Stem Cell Transcriptome Seal Menzorov, Aleksei Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
topic_facet |
Stem Cell Transcriptome Seal |
description |
We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis. |
format |
Dataset |
author |
Menzorov, Aleksei |
author_facet |
Menzorov, Aleksei |
author_sort |
Menzorov, Aleksei |
title |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_short |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_full |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_fullStr |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_full_unstemmed |
Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data |
title_sort |
ringed seal (phoca hispida) induced multipotent stem cells transcriptome analysis data |
publisher |
Mendeley |
publishDate |
2021 |
url |
https://dx.doi.org/10.17632/5mkvk5yc4w.2 https://data.mendeley.com/datasets/5mkvk5yc4w/2 |
genre |
Phoca hispida ringed seal |
genre_facet |
Phoca hispida ringed seal |
op_relation |
https://dx.doi.org/10.17632/5mkvk5yc4w |
op_rights |
Creative Commons Attribution 4.0 International info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17632/5mkvk5yc4w.2 https://doi.org/10.17632/5mkvk5yc4w |
_version_ |
1766167429560401920 |