Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ...
Polysaccharides represent a labile, abundant class of marine dissolved organic matter (DOM), which must be hydrolyzed by extracellular enzymes prior to uptake by heterotrophic microbes. Pelagic microbial communities differ in their ability to access polysaccharides: some communities are completely i...
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The University of North Carolina at Chapel Hill University Libraries
2009
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ftdatacite:10.17615/3zys-5043 2024-03-31T07:50:03+00:00 Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... Steen, Andrew D. 2009 https://dx.doi.org/10.17615/3zys-5043 https://cdr.lib.unc.edu/concern/dissertations/z603qx638?locale=en en eng The University of North Carolina at Chapel Hill University Libraries In Copyright http://rightsstatements.org/vocab/InC/1.0/ Text thesis Dissertation Thesis 2009 ftdatacite https://doi.org/10.17615/3zys-5043 2024-03-04T11:32:16Z Polysaccharides represent a labile, abundant class of marine dissolved organic matter (DOM), which must be hydrolyzed by extracellular enzymes prior to uptake by heterotrophic microbes. Pelagic microbial communities differ in their ability to access polysaccharides: some communities are completely incapable of accessing certain polysaccharides which are readily hydrolyzed elsewhere. I measured enzymatic hydrolysis of structurally defined polysaccharides in the Gulf of Mexico and in Svalbard fjords (Arctic Ocean), and investigated the factors that influenced enzyme activities. Arctic communities were able to access a narrower range of polysaccharide structures than Gulf of Mexico surface water communities, but Gulf of Mexico mesopelagic (>100 m depth) communities also accessed a limited range of polysaccharides. Attempts to induce expression of pullulanase, which was not present in Arctic samples, suggested that the Arctic communities were totally incapable of expressing pullulanase, most likely for lack ... Thesis Arctic Arctic Ocean Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Arctic Ocean Svalbard |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
description |
Polysaccharides represent a labile, abundant class of marine dissolved organic matter (DOM), which must be hydrolyzed by extracellular enzymes prior to uptake by heterotrophic microbes. Pelagic microbial communities differ in their ability to access polysaccharides: some communities are completely incapable of accessing certain polysaccharides which are readily hydrolyzed elsewhere. I measured enzymatic hydrolysis of structurally defined polysaccharides in the Gulf of Mexico and in Svalbard fjords (Arctic Ocean), and investigated the factors that influenced enzyme activities. Arctic communities were able to access a narrower range of polysaccharide structures than Gulf of Mexico surface water communities, but Gulf of Mexico mesopelagic (>100 m depth) communities also accessed a limited range of polysaccharides. Attempts to induce expression of pullulanase, which was not present in Arctic samples, suggested that the Arctic communities were totally incapable of expressing pullulanase, most likely for lack ... |
format |
Thesis |
author |
Steen, Andrew D. |
spellingShingle |
Steen, Andrew D. Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
author_facet |
Steen, Andrew D. |
author_sort |
Steen, Andrew D. |
title |
Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
title_short |
Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
title_full |
Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
title_fullStr |
Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
title_full_unstemmed |
Microbial degradation of organic macromolecules in Arctic fjords and in the Gulf of Mexico ... |
title_sort |
microbial degradation of organic macromolecules in arctic fjords and in the gulf of mexico ... |
publisher |
The University of North Carolina at Chapel Hill University Libraries |
publishDate |
2009 |
url |
https://dx.doi.org/10.17615/3zys-5043 https://cdr.lib.unc.edu/concern/dissertations/z603qx638?locale=en |
geographic |
Arctic Arctic Ocean Svalbard |
geographic_facet |
Arctic Arctic Ocean Svalbard |
genre |
Arctic Arctic Ocean Svalbard |
genre_facet |
Arctic Arctic Ocean Svalbard |
op_rights |
In Copyright http://rightsstatements.org/vocab/InC/1.0/ |
op_doi |
https://doi.org/10.17615/3zys-5043 |
_version_ |
1795028121719668736 |