Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ...
To provide an overview of the distribution of polar cod (Boreogadus saida) across the Bering and Chukchi Seas, environmental DNA (eDNA) of polar cod was analyzed using a novel species-specific assay. eDNA samples were collected on the cruise (MR20-05C) conducted by the 'R/V Mirai' of the J...
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Arctic Data archive System (ADS), Japan
2023
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Online Access: | https://dx.doi.org/10.17592/001.2023032302 https://ads.nipr.ac.jp/dataset/A20230323-002 |
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ftdatacite:10.17592/001.2023032302 2023-12-03T10:20:29+01:00 Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... Kawakami, Tatsuya Yamazaki, Aya Jiang, Hai-Chao Ueno, Hiromichi Kasai, Akihide 2023 https://dx.doi.org/10.17592/001.2023032302 https://ads.nipr.ac.jp/dataset/A20230323-002 en eng Arctic Data archive System (ADS), Japan Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 OCEANS>AQUATIC SCIENCES>FISHERIES BIOSPHERE>ECOSYSTEMS>MARINE ECOSYSTEMS>PELAGIC>OCEANIC ZONE dataset Dataset 2023 ftdatacite https://doi.org/10.17592/001.2023032302 2023-11-03T11:06:53Z To provide an overview of the distribution of polar cod (Boreogadus saida) across the Bering and Chukchi Seas, environmental DNA (eDNA) of polar cod was analyzed using a novel species-specific assay. eDNA samples were collected on the cruise (MR20-05C) conducted by the 'R/V Mirai' of the Japan Agency for Marine-Earth Science and Technology during the open-water season of 2020. For eDNA sampling, surface seawater was collected and then filtered through a cartridge filter (Sterivex HV, 0.45 m pore size; Millipore, Burlington, MA, USA). Additional eDNA samples were collected from the middle or bottom layer in the same manner. We developed a species-specific quantitative real-time PCR (qPCR) TaqMan assay to exclusively detect polar cod eDNA by targeting the D-loop region of mitochondrial DNA. Polar cod eDNA was detected and quantified in the samples using the assay. To investigate the relationship between eDNA detection and environmental conditions, temperature (degrees Celsius), salinity, chlorophyll-a ... Dataset Boreogadus saida Chukchi polar cod DataCite Metadata Store (German National Library of Science and Technology) Burlington ENVELOPE(-56.015,-56.015,49.750,49.750) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
OCEANS>AQUATIC SCIENCES>FISHERIES BIOSPHERE>ECOSYSTEMS>MARINE ECOSYSTEMS>PELAGIC>OCEANIC ZONE |
spellingShingle |
OCEANS>AQUATIC SCIENCES>FISHERIES BIOSPHERE>ECOSYSTEMS>MARINE ECOSYSTEMS>PELAGIC>OCEANIC ZONE Kawakami, Tatsuya Yamazaki, Aya Jiang, Hai-Chao Ueno, Hiromichi Kasai, Akihide Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
topic_facet |
OCEANS>AQUATIC SCIENCES>FISHERIES BIOSPHERE>ECOSYSTEMS>MARINE ECOSYSTEMS>PELAGIC>OCEANIC ZONE |
description |
To provide an overview of the distribution of polar cod (Boreogadus saida) across the Bering and Chukchi Seas, environmental DNA (eDNA) of polar cod was analyzed using a novel species-specific assay. eDNA samples were collected on the cruise (MR20-05C) conducted by the 'R/V Mirai' of the Japan Agency for Marine-Earth Science and Technology during the open-water season of 2020. For eDNA sampling, surface seawater was collected and then filtered through a cartridge filter (Sterivex HV, 0.45 m pore size; Millipore, Burlington, MA, USA). Additional eDNA samples were collected from the middle or bottom layer in the same manner. We developed a species-specific quantitative real-time PCR (qPCR) TaqMan assay to exclusively detect polar cod eDNA by targeting the D-loop region of mitochondrial DNA. Polar cod eDNA was detected and quantified in the samples using the assay. To investigate the relationship between eDNA detection and environmental conditions, temperature (degrees Celsius), salinity, chlorophyll-a ... |
format |
Dataset |
author |
Kawakami, Tatsuya Yamazaki, Aya Jiang, Hai-Chao Ueno, Hiromichi Kasai, Akihide |
author_facet |
Kawakami, Tatsuya Yamazaki, Aya Jiang, Hai-Chao Ueno, Hiromichi Kasai, Akihide |
author_sort |
Kawakami, Tatsuya |
title |
Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
title_short |
Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
title_full |
Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
title_fullStr |
Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
title_full_unstemmed |
Species-specific detection of polar cod (Boreogadus saida) eDNA from the Bering and Chukchi seas in the MR20-05C research cruise ... |
title_sort |
species-specific detection of polar cod (boreogadus saida) edna from the bering and chukchi seas in the mr20-05c research cruise ... |
publisher |
Arctic Data archive System (ADS), Japan |
publishDate |
2023 |
url |
https://dx.doi.org/10.17592/001.2023032302 https://ads.nipr.ac.jp/dataset/A20230323-002 |
long_lat |
ENVELOPE(-56.015,-56.015,49.750,49.750) |
geographic |
Burlington |
geographic_facet |
Burlington |
genre |
Boreogadus saida Chukchi polar cod |
genre_facet |
Boreogadus saida Chukchi polar cod |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.17592/001.2023032302 |
_version_ |
1784267908146266112 |