Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts
Osteoblast damage by oxidative stress has been recognized as a cause of bone-related disease, including osteoporosis. Recently, we reported that fermented Pacific oyster (Crassostrea gigas) extracts (FO) inhibited osteoclastogenesis and osteoporosis, while promoting osteogenesis. However, since the...
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IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
2020
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ftdatacite:10.17179/excli2020-2376 2023-05-15T15:58:58+02:00 Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts Park, Cheol Lee, Hyesook Han, Min Ho Jeong, Jin-Woo Kim, Sung Ok Jeong, Soon-Jeong Lee, Bae‐Jin Kim, Gi‐Young Park, Eui Kyun Jeon, You‐Jin Choi, Yung Hyun 2020 https://dx.doi.org/10.17179/excli2020-2376 https://www.excli.de/vol19/excli2020-2376.pdf en eng IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). CC-BY fermented oyster extract ROS DNA damage apoptosis Nrf2/HO-1 Text article-journal ScholarlyArticle 2020 ftdatacite https://doi.org/10.17179/excli2020-2376 2021-11-05T12:55:41Z Osteoblast damage by oxidative stress has been recognized as a cause of bone-related disease, including osteoporosis. Recently, we reported that fermented Pacific oyster (Crassostrea gigas) extracts (FO) inhibited osteoclastogenesis and osteoporosis, while promoting osteogenesis. However, since the beneficial potential of FO on osteoblasts is not well known, in the present study, we investigated the cytoprotective effect of FO against oxidative stress in MC3T3-E1 osteoblasts. Our results demonstrated that FO inhibited hydrogen peroxide (H2O2)-induced DNA damage and cytotoxicity through the rescue of mitochondrial function by blocking abnormal ROS accumulation. FO also prevented apoptosis by suppressing loss of mitochondrial membrane potential and cytosolic release of cytochrome c, decreasing the rate of Bax/Bcl-2 expression and reducing the activity of caspase-9 and caspase-3 in H2O2-stimulated MC3T3-E1 osteoblasts, suggesting that FO protected MC3T3-E1 osteoblasts from the induction of caspase dependent- and mitochondria-mediated apoptosis by oxidative stress. In addition, FO markedly promoted the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2), which was associated with the enhanced expression of heme oxygenase-1 (HO-1). However, inhibiting the expression of HO-1 by artificially blocking the expression of Nrf2 using siRNA significantly eliminated the protective effect of FO, indicating that FO activates the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts to protect against oxidative stress. Based on the present data, FO is thought to be useful as a potential therapeutic agent for the inhibition of oxidative stress in osteoblasts. : EXCLI Journal; 19:Doc1102; ISSN 1611-2156 Text Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
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DataCite Metadata Store (German National Library of Science and Technology) |
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language |
English |
topic |
fermented oyster extract ROS DNA damage apoptosis Nrf2/HO-1 |
spellingShingle |
fermented oyster extract ROS DNA damage apoptosis Nrf2/HO-1 Park, Cheol Lee, Hyesook Han, Min Ho Jeong, Jin-Woo Kim, Sung Ok Jeong, Soon-Jeong Lee, Bae‐Jin Kim, Gi‐Young Park, Eui Kyun Jeon, You‐Jin Choi, Yung Hyun Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
topic_facet |
fermented oyster extract ROS DNA damage apoptosis Nrf2/HO-1 |
description |
Osteoblast damage by oxidative stress has been recognized as a cause of bone-related disease, including osteoporosis. Recently, we reported that fermented Pacific oyster (Crassostrea gigas) extracts (FO) inhibited osteoclastogenesis and osteoporosis, while promoting osteogenesis. However, since the beneficial potential of FO on osteoblasts is not well known, in the present study, we investigated the cytoprotective effect of FO against oxidative stress in MC3T3-E1 osteoblasts. Our results demonstrated that FO inhibited hydrogen peroxide (H2O2)-induced DNA damage and cytotoxicity through the rescue of mitochondrial function by blocking abnormal ROS accumulation. FO also prevented apoptosis by suppressing loss of mitochondrial membrane potential and cytosolic release of cytochrome c, decreasing the rate of Bax/Bcl-2 expression and reducing the activity of caspase-9 and caspase-3 in H2O2-stimulated MC3T3-E1 osteoblasts, suggesting that FO protected MC3T3-E1 osteoblasts from the induction of caspase dependent- and mitochondria-mediated apoptosis by oxidative stress. In addition, FO markedly promoted the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2), which was associated with the enhanced expression of heme oxygenase-1 (HO-1). However, inhibiting the expression of HO-1 by artificially blocking the expression of Nrf2 using siRNA significantly eliminated the protective effect of FO, indicating that FO activates the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts to protect against oxidative stress. Based on the present data, FO is thought to be useful as a potential therapeutic agent for the inhibition of oxidative stress in osteoblasts. : EXCLI Journal; 19:Doc1102; ISSN 1611-2156 |
format |
Text |
author |
Park, Cheol Lee, Hyesook Han, Min Ho Jeong, Jin-Woo Kim, Sung Ok Jeong, Soon-Jeong Lee, Bae‐Jin Kim, Gi‐Young Park, Eui Kyun Jeon, You‐Jin Choi, Yung Hyun |
author_facet |
Park, Cheol Lee, Hyesook Han, Min Ho Jeong, Jin-Woo Kim, Sung Ok Jeong, Soon-Jeong Lee, Bae‐Jin Kim, Gi‐Young Park, Eui Kyun Jeon, You‐Jin Choi, Yung Hyun |
author_sort |
Park, Cheol |
title |
Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
title_short |
Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
title_full |
Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
title_fullStr |
Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
title_full_unstemmed |
Cytoprotective effects of fermented oyster extracts against oxidative stress-induced DNA damage and apoptosis through activation of the Nrf2/HO-1 signaling pathway in MC3T3-E1 osteoblasts |
title_sort |
cytoprotective effects of fermented oyster extracts against oxidative stress-induced dna damage and apoptosis through activation of the nrf2/ho-1 signaling pathway in mc3t3-e1 osteoblasts |
publisher |
IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund |
publishDate |
2020 |
url |
https://dx.doi.org/10.17179/excli2020-2376 https://www.excli.de/vol19/excli2020-2376.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_rights |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17179/excli2020-2376 |
_version_ |
1766394746100514816 |