Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches ...

• Main Authors: Jaarsma, Ate H., Sipes, Katie, Zervas, Athanasios, Campuzano Jiménez, Francisco, Ellegaard-Jensen, Lea, Thogersen, Mariane S., Stougaard, Peter, Benning, Liane G., Tranter, Martyn, Anesio, Alexandre M.
• Format: Text
• Language: unknown
• Published: Freie Universität Berlin 2023
• Subjects: amplicons; Greenland Ice Sheet; in situ culturing; isolates; MAGs; metagenome; 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie570 Biowissenschaften; Biologie; Greenland; Ice Sheet
• Online Access: https://dx.doi.org/10.17169/refubium-41177; https://refubium.fu-berlin.de/handle/fub188/41455

The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the ...