Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos
Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study,...
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Freie Universität Berlin
2021
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ftdatacite:10.17169/refubium-31573 2023-05-15T18:42:24+02:00 Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos Hein, Anna Baumgartner, Katrin Von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula 2021 https://dx.doi.org/10.17169/refubium-31573 https://refubium.fu-berlin.de/handle/fub188/31840 unknown Freie Universität Berlin https://doi.org/10.1016/j.ygcen.2021.113837 https://dx.doi.org/10.1016/j.ygcen.2021.113837 https://doi.org/10.1016/j.ygcen.2021.113837 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Cortisol Hair Steroid hormones Non-invasive LC–MS/MS Ursus maritimus 600 Technik, Medizin, angewandte Wissenschaften630 Landwirtschaft630 Landwirtschaft und verwandte Bereiche Text article-journal Wissenschaftlicher Artikel ScholarlyArticle 2021 ftdatacite https://doi.org/10.17169/refubium-31573 https://doi.org/10.1016/j.ygcen.2021.113837 2021-11-05T12:55:41Z Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study, hair samples of zoo-housed polar bears were analyzed for cortisol with two independent immunoassays [an enzyme-linked immunoassay (EIA) and a chemiluminescence assay (CLIA)] and liquid chromatography–tandem mass spectrometry (LC–MS/MS). HCC measurements depended significantly on assay type applied, sample processing (cutting vs. powdering hair) and their interaction. Best agreement was observed between LC–MS/MS and CLIA (R2 = 0.81 for powdered hair) and sample processing had a minor, albeit significant, effect on obtained HCC measurements in these assays (R2 > 0.9). EIA measurements were consistently higher than with the other assays. HCC measurement was validated biologically for CLIA and LC–MS/MS in one male polar bear that experienced considerable stress for a prolonged period of time (> 18 weeks). Subsequently, by using the validated LC–MS/MS the measurement of cortisol could be complemented by the analysis of other steroids including cortisone, testosterone and progesterone levels from hair samples collected over a 9-month period (5–13 months) from six zoo-housed polar bears (five males, one female). No seasonal steroid variation was observed except in male progesterone levels. For all steroids except cortisone, a strong body region effect (neck or paw) was observed. Cortisol and cortisone, as well as progesterone and testosterone, concentrations were positively correlated. We show that hair steroid concentrations can be used to longitudinally measure stress and reproductive hormone axes in polar bears. The data established herein provide important basic information regarding methodology and study design for assessing hair steroid hormones. Text Ursus maritimus DataCite Metadata Store (German National Library of Science and Technology) Eia ENVELOPE(7.755,7.755,63.024,63.024) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Cortisol Hair Steroid hormones Non-invasive LC–MS/MS Ursus maritimus 600 Technik, Medizin, angewandte Wissenschaften630 Landwirtschaft630 Landwirtschaft und verwandte Bereiche |
spellingShingle |
Cortisol Hair Steroid hormones Non-invasive LC–MS/MS Ursus maritimus 600 Technik, Medizin, angewandte Wissenschaften630 Landwirtschaft630 Landwirtschaft und verwandte Bereiche Hein, Anna Baumgartner, Katrin Von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
topic_facet |
Cortisol Hair Steroid hormones Non-invasive LC–MS/MS Ursus maritimus 600 Technik, Medizin, angewandte Wissenschaften630 Landwirtschaft630 Landwirtschaft und verwandte Bereiche |
description |
Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study, hair samples of zoo-housed polar bears were analyzed for cortisol with two independent immunoassays [an enzyme-linked immunoassay (EIA) and a chemiluminescence assay (CLIA)] and liquid chromatography–tandem mass spectrometry (LC–MS/MS). HCC measurements depended significantly on assay type applied, sample processing (cutting vs. powdering hair) and their interaction. Best agreement was observed between LC–MS/MS and CLIA (R2 = 0.81 for powdered hair) and sample processing had a minor, albeit significant, effect on obtained HCC measurements in these assays (R2 > 0.9). EIA measurements were consistently higher than with the other assays. HCC measurement was validated biologically for CLIA and LC–MS/MS in one male polar bear that experienced considerable stress for a prolonged period of time (> 18 weeks). Subsequently, by using the validated LC–MS/MS the measurement of cortisol could be complemented by the analysis of other steroids including cortisone, testosterone and progesterone levels from hair samples collected over a 9-month period (5–13 months) from six zoo-housed polar bears (five males, one female). No seasonal steroid variation was observed except in male progesterone levels. For all steroids except cortisone, a strong body region effect (neck or paw) was observed. Cortisol and cortisone, as well as progesterone and testosterone, concentrations were positively correlated. We show that hair steroid concentrations can be used to longitudinally measure stress and reproductive hormone axes in polar bears. The data established herein provide important basic information regarding methodology and study design for assessing hair steroid hormones. |
format |
Text |
author |
Hein, Anna Baumgartner, Katrin Von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula |
author_facet |
Hein, Anna Baumgartner, Katrin Von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula |
author_sort |
Hein, Anna |
title |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_short |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_full |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_fullStr |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_full_unstemmed |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_sort |
analysis of hair steroid hormones in polar bears (ursus maritimus) via liquid chromatography–tandem mass spectrometry: comparison with two immunoassays and application for longitudinal monitoring in zoos |
publisher |
Freie Universität Berlin |
publishDate |
2021 |
url |
https://dx.doi.org/10.17169/refubium-31573 https://refubium.fu-berlin.de/handle/fub188/31840 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_relation |
https://doi.org/10.1016/j.ygcen.2021.113837 https://dx.doi.org/10.1016/j.ygcen.2021.113837 https://doi.org/10.1016/j.ygcen.2021.113837 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17169/refubium-31573 https://doi.org/10.1016/j.ygcen.2021.113837 |
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1766232058459324416 |