Gene expression response to a nematode parasite in novel and native eel hosts
Invasive parasites are involved in population declines of new host species worldwide. The high susceptibilities observed in many novel hosts have been attributed to the lack of protective immunity to the parasites which native hosts acquired during their shared evolution. We experimentally infected...
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ftdatacite:10.17169/refubium-25703 2023-05-15T13:26:57+02:00 Gene expression response to a nematode parasite in novel and native eel hosts Bracamonte, Seraina E. Johnston, Paul R. Monaghan, Michael T. Knopf, Klaus 2019 https://dx.doi.org/10.17169/refubium-25703 https://refubium.fu-berlin.de/handle/fub188/25944 unknown Freie Universität Berlin https://doi.org/10.1002/ece3.5728 https://dx.doi.org/10.1002/ece3.5728 https://doi.org/10.1002/ece3.5728 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Anguilla anguilla Anguilla japonica Anguillicola crassus comparative transcriptomics emerging infectious disease host‐parasite interaction 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie576 Genetik und Evolution Text article-journal Wissenschaftlicher Artikel ScholarlyArticle 2019 ftdatacite https://doi.org/10.17169/refubium-25703 https://doi.org/10.1002/ece3.5728 2021-11-05T12:55:41Z Invasive parasites are involved in population declines of new host species worldwide. The high susceptibilities observed in many novel hosts have been attributed to the lack of protective immunity to the parasites which native hosts acquired during their shared evolution. We experimentally infected Japanese eels (Anguilla japonica) and European eels (Anguilla anguilla) with Anguillicola crassus, a nematode parasite that is native to the Japanese eel and invasive in the European eel. We inferred gene expression changes in head kidney tissue from both species, using RNA‐seq data to determine the responses at two time points during the early stages of infection (3 and 23 days postinfection). At both time points, the novel host modified the expression of a larger and functionally more diverse set of genes than the native host. Strikingly, the native host regulated immune gene expression only at the earlier time point and to a small extent while the novel host regulated these genes at both time points. A low number of differentially expressed immune genes, especially in the native host, suggest that a systemic immune response was of minor importance during the early stages of infection. Transcript abundance of genes involved in cell respiration was reduced in the novel host which may affect its ability to cope with harsh conditions and energetically demanding activities. The observed gene expression changes in response to a novel parasite that we observed in a fish follow a general pattern observed in amphibians and mammals, and suggest that the disruption of physiological processes, rather than the absence of an immediate immune response, is responsible for the higher susceptibility of the novel host. Text Anguilla anguilla European eel DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Anguilla anguilla Anguilla japonica Anguillicola crassus comparative transcriptomics emerging infectious disease host‐parasite interaction 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie576 Genetik und Evolution |
spellingShingle |
Anguilla anguilla Anguilla japonica Anguillicola crassus comparative transcriptomics emerging infectious disease host‐parasite interaction 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie576 Genetik und Evolution Bracamonte, Seraina E. Johnston, Paul R. Monaghan, Michael T. Knopf, Klaus Gene expression response to a nematode parasite in novel and native eel hosts |
topic_facet |
Anguilla anguilla Anguilla japonica Anguillicola crassus comparative transcriptomics emerging infectious disease host‐parasite interaction 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie576 Genetik und Evolution |
description |
Invasive parasites are involved in population declines of new host species worldwide. The high susceptibilities observed in many novel hosts have been attributed to the lack of protective immunity to the parasites which native hosts acquired during their shared evolution. We experimentally infected Japanese eels (Anguilla japonica) and European eels (Anguilla anguilla) with Anguillicola crassus, a nematode parasite that is native to the Japanese eel and invasive in the European eel. We inferred gene expression changes in head kidney tissue from both species, using RNA‐seq data to determine the responses at two time points during the early stages of infection (3 and 23 days postinfection). At both time points, the novel host modified the expression of a larger and functionally more diverse set of genes than the native host. Strikingly, the native host regulated immune gene expression only at the earlier time point and to a small extent while the novel host regulated these genes at both time points. A low number of differentially expressed immune genes, especially in the native host, suggest that a systemic immune response was of minor importance during the early stages of infection. Transcript abundance of genes involved in cell respiration was reduced in the novel host which may affect its ability to cope with harsh conditions and energetically demanding activities. The observed gene expression changes in response to a novel parasite that we observed in a fish follow a general pattern observed in amphibians and mammals, and suggest that the disruption of physiological processes, rather than the absence of an immediate immune response, is responsible for the higher susceptibility of the novel host. |
format |
Text |
author |
Bracamonte, Seraina E. Johnston, Paul R. Monaghan, Michael T. Knopf, Klaus |
author_facet |
Bracamonte, Seraina E. Johnston, Paul R. Monaghan, Michael T. Knopf, Klaus |
author_sort |
Bracamonte, Seraina E. |
title |
Gene expression response to a nematode parasite in novel and native eel hosts |
title_short |
Gene expression response to a nematode parasite in novel and native eel hosts |
title_full |
Gene expression response to a nematode parasite in novel and native eel hosts |
title_fullStr |
Gene expression response to a nematode parasite in novel and native eel hosts |
title_full_unstemmed |
Gene expression response to a nematode parasite in novel and native eel hosts |
title_sort |
gene expression response to a nematode parasite in novel and native eel hosts |
publisher |
Freie Universität Berlin |
publishDate |
2019 |
url |
https://dx.doi.org/10.17169/refubium-25703 https://refubium.fu-berlin.de/handle/fub188/25944 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
https://doi.org/10.1002/ece3.5728 https://dx.doi.org/10.1002/ece3.5728 https://doi.org/10.1002/ece3.5728 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17169/refubium-25703 https://doi.org/10.1002/ece3.5728 |
_version_ |
1766395521947140096 |