Reference genes for quantitative gene expression studies in multiple avian species

• Main Authors: Olias, Philipp, Adam, Iris, Meyer, Anne, Scharff, Constance, Gruber, Achim D.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Freie Universität Berlin 2014
• Subjects: zebra finch; gene expression; RNA extraction; RNA synthesis; 500 Naturwissenschaften und Mathematik570 Biowissenschaften; Biologie576 Genetik und Evolution; Finch; Haliaeetus albicilla; White-tailed eagle
• Online Access: https://dx.doi.org/10.17169/refubium-25473; https://refubium.fu-berlin.de/handle/fub188/25709

Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data. In recent years, an increasing number of avian s...