Seawater carbonate chemistry and oxidative stress response, carbon metabolism and rapid light curve fitted parameters of phytoplankton Phaeodactylum tricornutum ...

• Main Authors: Moreno, Hugo, Rokitta, Sebastian D, Tremblay, Nelly, Boersma, Maarten, Groß, Elisabeth, Klip, Helena, Wiltshire, Karen Helen, Meunier, Cédric Léo
• Format: Dataset
• Language: unknown
• Published: PANGAEA 2024
• Subjects: Bacillariophyta; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Not applicable; Other metabolic rates; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Temperature; Type of study; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Strain; Treatment temperature; Treatment partial pressure of carbon dioxide; Nitrogen/Phosphorus ratio; Replicate; Growth rate; Carbon per cell; Phosphorus, organic, particulate, production per cell; Respiration rate, carbon; Carbon, organic, dissolved exudation, per cell; Superoxide dismutase manganese activity, unit per protein mass; Glutathione peroxidase activity, unit per protein mass; Glutathione S-transferase activity, unit per protein mass; Catalase activity, unit per protein mass; Malondialdehyde, per wet mass; Chlorophyll a per cell; Fucoxanthin per cell; beta-Carotene per cell; Diadinoxanthin per cell; Violaxanthin per cell; Initial slope of rapid light curve; Initial slope of rapid light curve, standard deviation; Compensation point; Compensation point, standard deviation; Net photosynthesis rate, oxygen, per chlorophyll a; Net photosynthesis rate, standard deviation; Light saturation point; Light saturation point, standard deviation; Chlorophyll a, standard deviation; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.966813; https://doi.pangaea.de/10.1594/PANGAEA.966813

We conducted a full-factorial lab experiment to study the individual and combined effects of temperature (18°C and 21°C), pCO2 (400 and 1000 ppm), and dissolved N:P ratio (16 and 25 molar) on the antioxidant capacity and carbon metabolism of the phytoplankton Phaeodactylum tricornutum (strain CCAP 1052/1A). The antioxidant response was assessed based on different biomarkers, including the contents of protective carotenoids (ß-Carotene, diadinoxanthin and violaxanthin), and by determination of antioxidant enzyme activities, and Malondialdehyde (MDA) as a biomarker for oxidative stress. We quantified the activity of Managanese Superoxide Dismutase (SOD-Mn), Glutathione S-transferase (GST), Catalase (CAT), Glutathione Peroxidase (GPx). As possible consequence of oxidative stress on metabolic pathways of carbon, we also quantified carbon fluxes by measuring rates of growth, respiration, dissolved organic carbon (DOC) exudation, and cellular organic carbon content and particulate phosphorus (PP). We also ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2024) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2024-03-20. ...