Cellular stress biomarkers result of the cold-water coral Dentomuricea aff. meteor during a land-based experiment testing the cumulative effect of Cu exposure and ocean acidification ...

• Main Authors: Martins, Ines, Goulart, Joana, Godinho, Antonio, Mano, Beatriz, Sire de Vilar, Anaïs, Carreiro-Silva, Marina
• Format: Dataset
• Language: unknown
• Published: PANGAEA 2024
• Subjects: Experiment; Species; Species, unique identification Semantic URI; Species, unique identification URI; Treatment; Time in days; Aquaria ID; Colony number/ID; Glutathione S-transferase, activity, per protein mass; Superoxide dismutase activity, unit per protein mass; Carbonic anhydrase activity, per protein; Heat shock protein 70, per protein mass; Malondialdehyde, per protein mass; Laboratory experiment; Multiskan Microplate spectrophotometer, Thermo Scientific™, GO; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time iAtlantic; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.966792; https://doi.pangaea.de/10.1594/PANGAEA.966792

We report the results of a land-based experiment testing the cumulative effects of copper (Cu) exposure and ocean acidification (OA). Corals were obtained as by-catch during experimental long-line fisheries campaigns on board R/V "Arquipélago" (ARQDAÇO monitoring program, University of the Azores) at Baixa de São Mateus at 185 m depth in April 2019. The experiment was undertaken at the DeepSeaLab aquaria facilities (Okeanos-University of the Azores) where corals were exposed to four OA/Cu-contamination scenarios: (1) ambient pCO2/pH level as measured in situ conditions (385 μatm/ pH 8.09); (2) high pCO2/reduced pH (IPCC RCP8.5 scenario, 1000 μatm/ pH 7.73); (3) ambient pCO2/pH level and additional Cu concentration (60 µg/l); (4) high pCO2/reduced pH and additional Cu concentration (60 µg/l). The pH/pCO2 modification was achieved by bubbling seawater with either pure CO2 (to increase pCO2) or CO2 low air (to decrease pCO2). The copper concentration used in this experiment followed a trial simulating a ... : Species: octocoral Dentomuricea aff. meteor1. Experimental treatmentsTreatments8.1 + [0]: ambient pH plus no copper addition8.1 + [60]: ambient pH plus Cu concentration of 60 μg/l7.7 + [0]: low pH plus no copper addition7.7 + [60]: low pH plus Cu concentration of 60 μg/lExperiment duration: 9 days ...