Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ...
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed spo...
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2024
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Online Access: | https://dx.doi.org/10.1594/pangaea.966335 https://doi.pangaea.de/10.1594/PANGAEA.966335 |
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ftdatacite:10.1594/pangaea.966335 2024-06-09T07:38:48+00:00 Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... Leys, Sally P Federwisch, Luisa 2024 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.966335 https://doi.pangaea.de/10.1594/PANGAEA.966335 unknown PANGAEA https://dx.doi.org/10.1594/pangaea.965868 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Antarctic Demospongiae Hexactinellida JR17003a microbes microbiome microscopy Sponges TEM transmission electron microscopy ultrastructure Weddell Sea Event label Latitude of event Longitude of event Elevation of event Sample ID Image number/name Image Agassiz Trawl Transmission electron microscope TEM JR17003A James Clark Ross Bentho-Pelagic Processes @ AWI AWI_BPP Dataset dataset 2024 ftdatacite https://doi.org/10.1594/pangaea.96633510.1594/pangaea.965868 2024-05-13T13:07:05Z This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. ... Dataset Antarc* Antarctic Weddell Sea DataCite Metadata Store (German National Library of Science and Technology) Antarctic Prince Gustav Channel ENVELOPE(-58.250,-58.250,-63.833,-63.833) Weddell Weddell Sea |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Antarctic Demospongiae Hexactinellida JR17003a microbes microbiome microscopy Sponges TEM transmission electron microscopy ultrastructure Weddell Sea Event label Latitude of event Longitude of event Elevation of event Sample ID Image number/name Image Agassiz Trawl Transmission electron microscope TEM JR17003A James Clark Ross Bentho-Pelagic Processes @ AWI AWI_BPP |
spellingShingle |
Antarctic Demospongiae Hexactinellida JR17003a microbes microbiome microscopy Sponges TEM transmission electron microscopy ultrastructure Weddell Sea Event label Latitude of event Longitude of event Elevation of event Sample ID Image number/name Image Agassiz Trawl Transmission electron microscope TEM JR17003A James Clark Ross Bentho-Pelagic Processes @ AWI AWI_BPP Leys, Sally P Federwisch, Luisa Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
topic_facet |
Antarctic Demospongiae Hexactinellida JR17003a microbes microbiome microscopy Sponges TEM transmission electron microscopy ultrastructure Weddell Sea Event label Latitude of event Longitude of event Elevation of event Sample ID Image number/name Image Agassiz Trawl Transmission electron microscope TEM JR17003A James Clark Ross Bentho-Pelagic Processes @ AWI AWI_BPP |
description |
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. ... |
format |
Dataset |
author |
Leys, Sally P Federwisch, Luisa |
author_facet |
Leys, Sally P Federwisch, Luisa |
author_sort |
Leys, Sally P |
title |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
title_short |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
title_full |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
title_fullStr |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
title_full_unstemmed |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea ... |
title_sort |
ultrastructure micrographs of antarctic deep-water sponges collected for microbiome study during expedition jr17003a in the western weddell sea ... |
publisher |
PANGAEA |
publishDate |
2024 |
url |
https://dx.doi.org/10.1594/pangaea.966335 https://doi.pangaea.de/10.1594/PANGAEA.966335 |
long_lat |
ENVELOPE(-58.250,-58.250,-63.833,-63.833) |
geographic |
Antarctic Prince Gustav Channel Weddell Weddell Sea |
geographic_facet |
Antarctic Prince Gustav Channel Weddell Weddell Sea |
genre |
Antarc* Antarctic Weddell Sea |
genre_facet |
Antarc* Antarctic Weddell Sea |
op_relation |
https://dx.doi.org/10.1594/pangaea.965868 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.96633510.1594/pangaea.965868 |
_version_ |
1801375447355752448 |