Flow cytometry dataset from CTD casts showing the abundance of microorganisms (smaller than 20 µm) during the Arctic MOSAiC expedition ...

• Main Authors: Müller, Oliver, Fong, Allison A, Snoeijs-Leijonmalm, Pauline, Creamean, Jessie, Olsen, Lasse Mørk, Eggers, Lena, Grosse, Julia, Hoppe, Clara Jule Marie, Torstensson, Anders, Heitman, Laura, Balmonte, John Paul, Chamberlain, Emelia, Dietrich, Ulrike, Larsen, Aud, Petelenz, Elzbieta, Tsagaraki, Tatiana M, Mayers, Kyle, Langvad, Maria, Granskog, Mats A, Bratbak, Gunnar
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Arctic; flow cytometry; microbial abundance; MOSAiC_ECO; MOSAiC expedition; Water Column Data; Event label; Sample code/label; Leg Number; Activity description; Cast number; DATE/TIME; Device type; Depth, bathymetric; LATITUDE; LONGITUDE; Collector; Feature; DEPTH, water; Red only fluorescing picophytoplankton; Red only fluorescing nanophytoplankton; Red only fluorescing nanophytoplankton, small; Red only fluorescing nanophytoplankton, large; Orange and red fluorescing picophytoplankton; Orange and red fluorescing nanophytoplankton; Orange fluorescing prokaryote picophytoplankton; Heterotrophic nanophytoplankton; Heterotrophic prokaryotes; Heterotrophic prokaryotes with relatively low nucleic acid; Heterotrophic prokaryotes with relatively high nucleic acid; Heterotrophic prokaryotes, medium; Heterotrophic prokaryotes, large; Heterotrophic prokaryotes, very large; Virus-like particles; Virus, low DNA fluorescence; Virus, medium DNA fluorescence; Virus, high DNA fluorescence; CTD/Rosette; Acoustic focusing cytometer, Thermo Fisher, Attune NxT [20 mW 488 nm blue laser]; Flow cytometry system, Becton Dickinson, FACSCalibur; PS122/1; PS122/2; PS122/3; PS122/4; PS122/5; Polarstern; Multidisciplinary drifting Observatory for the Study of Arctic Climate MOSAiC; Ridges - Safe HAVens for ice-associated Flora and Fauna in a Seasonally ice-covered Arctic OCean HAVOC; Arctic Ocean
• Online Access: https://dx.doi.org/10.1594/pangaea.963430; https://doi.pangaea.de/10.1594/PANGAEA.963430

This dataset gives an overview of the abundance of microorganisms (smaller than 20 µm) enumerated using flow cytometry (FCM) during the Multidisciplinary drifting observatory for the study of Arctic Climate (MOSAiC) sampled from ship-based and on-ice CTD rosettes during leg 1, 2, 3, 4 and 5 (November 2019 – September 2020). Additional expedition and sampling details can be found in the ECO-overview paper (Fong et al., to be submitted to Elementa). We thank all persons involved in the expedition of the Research Vessel Polarstern during MOSAiC in 2019-2020 (AWI_PS122_00) as listed in Nixdorf et al. (2021).Flow cytometry (FCM) is a fast, high-throughput method to enumerate the abundance of microorganism (smaller than 20 µm). FCM uses the hydrodynamic focusing of a laminar flow to separate and line up microscopic particles. When particles pass a laser beam, the generated light scattering can be used to estimate their cell size, obtain information about cell granularity and surface characteristics and determine ...