Water record of Magallana angulata F0 generation exposed to low pH during laboratory experiments in 2020 ...

• Main Author: Dang, Xin
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Crassostrea angulata; Laboratory experiment; Ocean acidification; Transgenerational effect; Type of study; Date/time start, experiment; Date/time end, experiment; Sampling date/time, experiment; Species, unique identification; Species, unique identification Semantic URI; Species, unique identification URI; Experimental treatment; Chamber number; Temperature, water; Salinity; Treatment pH; Alkalinity, total; Carbon dioxide, partial pressure; Carbonate ion; Calcite saturation state; Aragonite saturation state; Sampling by hand; pH meter, Mettler Toledo, Pro2Go Portable; Refractometer, Mingrui, LS10T; Alkalinity titrator Mettler Toledo G20S Compact Titrator, Switzerland; Calculated using CO2SYS program Pierrot et al. 2012
• Online Access: https://dx.doi.org/10.1594/pangaea.962532; https://doi.pangaea.de/10.1594/PANGAEA.962532

The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the hatchery located in Zhanjiang, China (21.07°N, 110.74°E). The two-year-old Portuguese oysters Magallana angulata (also known as Crassostrea angulata) were collected from Wuchuan, China (21.37°N, 110.74°E). To be specific, about 300 adult oysters were transferred into six black fibreglass tanks (500L each) randomly and each culture tank contained about 50 individuals. Six tanks were independent and completely randomized to be separated into two different pH levels: ambient pH 8.0~8.1 as control (named F0_8.1) and pH 7.4 as ocean acidification (OA) treatment (named F0_7.4) for about one month to produce next generation and test for transgenerational effect. Low pH was driven by bubbling of carbon dioxide (CO₂) enriched air mixed with ambient air into filter seawater in the treatment tanks. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab®, ...