Metadata for incubation experiment testing temperature effects on a microbial community from Fram Strait, June 2021 ...

• Main Authors: Ahme, Antonia, von Jackowski, Anabel, McPherson, Rebecca, Wolf, Klara K E, Hoppmann, Mario, Neuhaus, Stefan, Kühne, Nancy, John, Uwe
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Arctic; Bacteria; cell size; Fram Strait; incubation experiment; Laboratory experiment; Microplankton; Pelagic microorganisms; Phytoplankton; protists; species composition; Temperature; thermal tolerance; traits; trophic state; West Spitsbergen Current; Event label; Type of study; Laboratory; Date/time start, experiment; Date/time end, experiment; Sample code/label; Treatment: temperature; Replicate; Time point, descriptive; pH; Alkalinity, total; Carbon dioxide, partial pressure; Bicarbonate ion; Carbon trioxide; Carbon dioxide, dissolved; Carbon, inorganic, dissolved; Chlorophyll a; Carbon, organic, particulate; Nitrogen, organic, particulate; Phosphate; Silicate; Nitrite; Nitrate; Ammonium; Multiparameter probe CTD, Sea-Bird, SBE 911plus; coupled with Carousel Water Sampler, Sea-Bird, SBE 32; pH meter, Thermo Scientific, EcoScan PH5; coupled with pH glass electrode, Mettler Toledo, Columbus; Titration analyzer, Schott Instruments, TitroLine alpha plus; calculated from carbonate chemistry using the CO2Sys Excel sheet Pierrot, Lewis & Wallace, 2006; Laboratory fluorometer, Turner, Trilogy; Carbon and hydrogen and nitrogen and sulfur and oxygen CHNSO elemental analyzer, EuroVector, EA3000; Continuous flow autoanalyzer, Alliance Instruments, Evolution III; PS126; Polarstern; Changing Earth – Sustaining our Future Helmholtz_ChangingEarth; Arctic Ocean; Schott; Toledo; Spitsbergen
• Online Access: https://dx.doi.org/10.1594/pangaea.960624; https://doi.pangaea.de/10.1594/PANGAEA.960624

We performed a temperature incubation experiment on board the RV Polarstern with a unicellular microbial community sampled from the Hausgarten station IV in Fram Strait during the campagin PS126 on June 1st, 2021 (Soltwedel et al., 2021). The community was sampled with CTD-bound niskin bottles (SBE 32 Carousel Water Sampler attached to a Seabird SBE911+ CTD-system; Seabird Scientific, Bellevue, WA, USA) from a depth of 15 m (Hoppmann et al., in review) and, after filtering the seawater through a 150 µm net, incubated in triplicate on plankton wheels in three temperature-controlled containers for ten days. To mimick todays and potential future temperature conditions of the Arctic ocean, we chose a control temperature of 2 °C, an intermediate warming scenario of 6 °C, and an extreme warming scenario of 9 °C. The goal was to investigate the effects of concurrent warming and Atlantification and therefore we chose an Arctic-Atlantic mixed water mass as community origin. This dataset comprises the chlorophyll, ...