Physiological measurements from the long-term laboratory incubation of Emiliania huxleyi grown at present day temperature and pH conditions in subantarctic waters and projected conditions for 2100 ...

• Main Authors: Armstrong, Evelyn, Law, Cliff S
• Format: Article in Journal/Newspaper
• Language: English
• Published: PANGAEA 2023
• Subjects: adaptation; Emiliania huxleyi; Laboratory experiment; Ocean acidification; ocean warming; subantarctic; Currie; New Zealand
• Online Access: https://dx.doi.org/10.1594/pangaea.959857; https://doi.pangaea.de/10.1594/PANGAEA.959857

Lower pH and elevated temperature alter phytoplankton growth and biomass in short-term incubations, but longer-term responses and adaptation potential are less well-studied. To determine the future of the coccolithophore Emiliania huxleyi, in subantarctic waters, a mixed genotype culture was incubated for two years. E. huxleyi was isolated from subantarctic waters at the end of the Munida Transect (Currie et al. 2011) east of New Zealand (-45.829 171.532) on 3rd June 2014. The coccolithophore was isolated in Aquil medium but then maintained in a 10-fold dilution of the recommended addition of Guillards f/2 (Sigma G0154) to natural seawater supplemented with additional nutrients to nitrate 96 μM and phosphate 6 μM (f/20). In this medium, the strain continued to calcify throughout the experiment after treatment with the antibiotics penicillin, streptomycin and neomycin (Sigma P4083) at the recommended dosage to remove bacteria prior to the start of the incubation (20th November 2015). E. huxleyi was incubated ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ...