Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ...

• Main Authors: Armstrong, Evelyn, Law, Cliff S
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: adaptation; Emiliania huxleyi; Laboratory experiment; Ocean acidification; ocean warming; subantarctic; Species, unique identification; Species, unique identification Semantic URI; Species, unique identification URI; Type of study; Time in days; Treatment; Chlorophyll a per cell; Carbon, organic, particulate, per cell; Nitrogen, organic, particulate, per cell; Phosphorus, organic, particulate, per cell; Carbon, inorganic, particulate, per cell; Calcium uptake, per cell; Carbon uptake, per cell; Calcium/Carbon uptake ratio; Carbon, organic, particulate/chlorophyll a ratio; Particulate inorganic carbon/particulate organic carbon ratio; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Phosphorus, organic, particulate ratio; Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio; Chlorophyll a; Carbon, organic, particulate; Nitrogen, organic, particulate; Phosphorus, organic, particulate; Carbon, inorganic, particulate; Cell surface area; Bottle, Niskin; Fluorometer, Turner Designs, 10-AU; CHN elemental analyser, Thermo Fisher Scientific, FLASH 2000; Measured according to Solorzano and Sharp 1980; Calculated; P140603; Polaris II
• Online Access: https://dx.doi.org/10.1594/pangaea.959839; https://doi.pangaea.de/10.1594/PANGAEA.959839

During a two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), at the final cross-over point (Day 670) an additional suite of measurements were performed with cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future). For in vitro chlorophyll a (chl-a) measurement, chl-a was extracted from the cells for 20 hours in 90 % acetone and the fluorescence, before and after acidification, measured using a Turner 10-AU fluorometer (Parsons et al. 1984; Welschmeyer 1994). Particulate organic carbon (POC) and particulate organic nitrogen (PON) cell content was determined in cells collected on pre-ashed GF/F (Whatman) filters with particulate inorganic carbon (PIC) removed by flooding the filter with 0.1N sulphuric acid on the filtration manifold (Kennedy et al. 2005). ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ...