Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ...
During a two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), at the final cross-over point (Day 670) an additional suite of measurements were performed with cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - F...
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ftdatacite:10.1594/pangaea.959839 2024-06-09T07:48:49+00:00 Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... Armstrong, Evelyn Law, Cliff S 2023 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.959839 https://doi.pangaea.de/10.1594/PANGAEA.959839 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.959857 https://dx.doi.org/10.1371/journal.pone.0284415 https://dx.doi.org/10.1002/rcm.1889 https://dx.doi.org/10.1007/s10126-008-9147-0 https://dx.doi.org/10.4319/lo.1980.25.4.0754 https://dx.doi.org/10.4319/lo.1994.39.8.1985 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Time in days Treatment Chlorophyll a per cell Carbon, organic, particulate, per cell Nitrogen, organic, particulate, per cell Phosphorus, organic, particulate, per cell Carbon, inorganic, particulate, per cell Calcium uptake, per cell Carbon uptake, per cell Calcium/Carbon uptake ratio Carbon, organic, particulate/chlorophyll a ratio Particulate inorganic carbon/particulate organic carbon ratio Carbon, organic, particulate/Nitrogen, organic, particulate ratio Carbon, organic, particulate/Phosphorus, organic, particulate ratio Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio Chlorophyll a Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Carbon, inorganic, particulate Cell surface area Bottle, Niskin Fluorometer, Turner Designs, 10-AU CHN elemental analyser, Thermo Fisher Scientific, FLASH 2000 Measured according to Solorzano and Sharp 1980 Calculated P140603 Polaris II Dataset dataset 2023 ftdatacite https://doi.org/10.1594/pangaea.95983910.1594/pangaea.95985710.1371/journal.pone.028441510.1002/rcm.188910.1007/s10126-008-9147-010.4319/lo.1980.25.4.075410.4319/lo.1994.39.8.1985 2024-05-13T13:07:02Z During a two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), at the final cross-over point (Day 670) an additional suite of measurements were performed with cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future). For in vitro chlorophyll a (chl-a) measurement, chl-a was extracted from the cells for 20 hours in 90 % acetone and the fluorescence, before and after acidification, measured using a Turner 10-AU fluorometer (Parsons et al. 1984; Welschmeyer 1994). Particulate organic carbon (POC) and particulate organic nitrogen (PON) cell content was determined in cells collected on pre-ashed GF/F (Whatman) filters with particulate inorganic carbon (PIC) removed by flooding the filter with 0.1N sulphuric acid on the filtration manifold (Kennedy et al. 2005). ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ... Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Time in days Treatment Chlorophyll a per cell Carbon, organic, particulate, per cell Nitrogen, organic, particulate, per cell Phosphorus, organic, particulate, per cell Carbon, inorganic, particulate, per cell Calcium uptake, per cell Carbon uptake, per cell Calcium/Carbon uptake ratio Carbon, organic, particulate/chlorophyll a ratio Particulate inorganic carbon/particulate organic carbon ratio Carbon, organic, particulate/Nitrogen, organic, particulate ratio Carbon, organic, particulate/Phosphorus, organic, particulate ratio Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio Chlorophyll a Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Carbon, inorganic, particulate Cell surface area Bottle, Niskin Fluorometer, Turner Designs, 10-AU CHN elemental analyser, Thermo Fisher Scientific, FLASH 2000 Measured according to Solorzano and Sharp 1980 Calculated P140603 Polaris II |
spellingShingle |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Time in days Treatment Chlorophyll a per cell Carbon, organic, particulate, per cell Nitrogen, organic, particulate, per cell Phosphorus, organic, particulate, per cell Carbon, inorganic, particulate, per cell Calcium uptake, per cell Carbon uptake, per cell Calcium/Carbon uptake ratio Carbon, organic, particulate/chlorophyll a ratio Particulate inorganic carbon/particulate organic carbon ratio Carbon, organic, particulate/Nitrogen, organic, particulate ratio Carbon, organic, particulate/Phosphorus, organic, particulate ratio Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio Chlorophyll a Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Carbon, inorganic, particulate Cell surface area Bottle, Niskin Fluorometer, Turner Designs, 10-AU CHN elemental analyser, Thermo Fisher Scientific, FLASH 2000 Measured according to Solorzano and Sharp 1980 Calculated P140603 Polaris II Armstrong, Evelyn Law, Cliff S Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
topic_facet |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Time in days Treatment Chlorophyll a per cell Carbon, organic, particulate, per cell Nitrogen, organic, particulate, per cell Phosphorus, organic, particulate, per cell Carbon, inorganic, particulate, per cell Calcium uptake, per cell Carbon uptake, per cell Calcium/Carbon uptake ratio Carbon, organic, particulate/chlorophyll a ratio Particulate inorganic carbon/particulate organic carbon ratio Carbon, organic, particulate/Nitrogen, organic, particulate ratio Carbon, organic, particulate/Phosphorus, organic, particulate ratio Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio Chlorophyll a Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Carbon, inorganic, particulate Cell surface area Bottle, Niskin Fluorometer, Turner Designs, 10-AU CHN elemental analyser, Thermo Fisher Scientific, FLASH 2000 Measured according to Solorzano and Sharp 1980 Calculated P140603 Polaris II |
description |
During a two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), at the final cross-over point (Day 670) an additional suite of measurements were performed with cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future). For in vitro chlorophyll a (chl-a) measurement, chl-a was extracted from the cells for 20 hours in 90 % acetone and the fluorescence, before and after acidification, measured using a Turner 10-AU fluorometer (Parsons et al. 1984; Welschmeyer 1994). Particulate organic carbon (POC) and particulate organic nitrogen (PON) cell content was determined in cells collected on pre-ashed GF/F (Whatman) filters with particulate inorganic carbon (PIC) removed by flooding the filter with 0.1N sulphuric acid on the filtration manifold (Kennedy et al. 2005). ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ... |
format |
Dataset |
author |
Armstrong, Evelyn Law, Cliff S |
author_facet |
Armstrong, Evelyn Law, Cliff S |
author_sort |
Armstrong, Evelyn |
title |
Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
title_short |
Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
title_full |
Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
title_fullStr |
Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
title_full_unstemmed |
Cell elemental contents and uptake rates of Emiliania huxleyi during laboratory experiments ... |
title_sort |
cell elemental contents and uptake rates of emiliania huxleyi during laboratory experiments ... |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://dx.doi.org/10.1594/pangaea.959839 https://doi.pangaea.de/10.1594/PANGAEA.959839 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1594/pangaea.959857 https://dx.doi.org/10.1371/journal.pone.0284415 https://dx.doi.org/10.1002/rcm.1889 https://dx.doi.org/10.1007/s10126-008-9147-0 https://dx.doi.org/10.4319/lo.1980.25.4.0754 https://dx.doi.org/10.4319/lo.1994.39.8.1985 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.95983910.1594/pangaea.95985710.1371/journal.pone.028441510.1002/rcm.188910.1007/s10126-008-9147-010.4319/lo.1980.25.4.075410.4319/lo.1994.39.8.1985 |
_version_ |
1801380723867779072 |