Growth rate over time of Emiliania huxleyi during laboratory experiments ...

• Main Authors: Armstrong, Evelyn, Law, Cliff S
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: adaptation; Emiliania huxleyi; Laboratory experiment; Ocean acidification; ocean warming; subantarctic; Species, unique identification; Species, unique identification Semantic URI; Species, unique identification URI; Type of study; Treatment; Time in days; Emiliania huxleyi, growth rate; Bottle, Niskin; Fluorometer, Turner Designs, 10-AU; P140603; Polaris II
• Online Access: https://dx.doi.org/10.1594/pangaea.959829; https://doi.pangaea.de/10.1594/PANGAEA.959829

Emiliania huxleyi was incubated for two years (2015-11-20 to 2017-12-01) under two conditions: present-day subantarctic water during summer (11°C and pH 8.1; Now), and projected conditions for subantarctic water by 2100 (14°C and pH 7.8; Future; Law et al. 2018). The pH was reduced by 0.3 units by bubbling 10% CO₂ through the medium prior to the addition of coccolithophore cells and confirmed using a spectrophotometric method (DOE 1994). One culture of each treatment was maintained in a semi-batch style to ensure the cells remained in exponential growth, with cell concentration maintained at low levels (<80000 cells ml⁻¹) to maintain pH at the target value. The pH of the medium after cell growth was checked periodically using a spectrophotometric method (DOE 1994) and was always found to be at the target pH. The in vivo fluorescence of the cultures was measured with a Turner 10-AU fluorometer and the growth rate of the cultures was calculated from the least squares regression of the natural logarithm of ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ...