Growth rate over time of Emiliania huxleyi during laboratory experiments ...
Emiliania huxleyi was incubated for two years (2015-11-20 to 2017-12-01) under two conditions: present-day subantarctic water during summer (11°C and pH 8.1; Now), and projected conditions for subantarctic water by 2100 (14°C and pH 7.8; Future; Law et al. 2018). The pH was reduced by 0.3 units by b...
Main Authors: | , |
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Format: | Dataset |
Language: | English |
Published: |
PANGAEA
2023
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.959829 https://doi.pangaea.de/10.1594/PANGAEA.959829 |
Summary: | Emiliania huxleyi was incubated for two years (2015-11-20 to 2017-12-01) under two conditions: present-day subantarctic water during summer (11°C and pH 8.1; Now), and projected conditions for subantarctic water by 2100 (14°C and pH 7.8; Future; Law et al. 2018). The pH was reduced by 0.3 units by bubbling 10% CO₂ through the medium prior to the addition of coccolithophore cells and confirmed using a spectrophotometric method (DOE 1994). One culture of each treatment was maintained in a semi-batch style to ensure the cells remained in exponential growth, with cell concentration maintained at low levels (<80000 cells ml⁻¹) to maintain pH at the target value. The pH of the medium after cell growth was checked periodically using a spectrophotometric method (DOE 1994) and was always found to be at the target pH. The in vivo fluorescence of the cultures was measured with a Turner 10-AU fluorometer and the growth rate of the cultures was calculated from the least squares regression of the natural logarithm of ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ... |
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