Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ...
On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and F...
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Online Access: | https://dx.doi.org/10.1594/pangaea.959827 https://doi.pangaea.de/10.1594/PANGAEA.959827 |
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ftdatacite:10.1594/pangaea.959827 2024-09-09T20:01:36+00:00 Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... Armstrong, Evelyn Law, Cliff S 2023 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.959827 https://doi.pangaea.de/10.1594/PANGAEA.959827 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.959857 https://dx.doi.org/10.1371/journal.pone.0284415 https://dx.doi.org/10.4319/lo.2008.53.3.1181 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Comment Treatment Time in days Emiliania huxleyi, cell biovolume Bottle, Niskin Inverted tissue culture microscope, Olympus, IX70 P140603 Polaris II Dataset dataset 2023 ftdatacite https://doi.org/10.1594/pangaea.95982710.1594/pangaea.95985710.1371/journal.pone.028441510.4319/lo.2008.53.3.1181 2024-07-03T10:20:00Z On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical. ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ... Dataset Ocean acidification DataCite Olympus ENVELOPE(156.767,156.767,-80.217,-80.217) |
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language |
English |
topic |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Comment Treatment Time in days Emiliania huxleyi, cell biovolume Bottle, Niskin Inverted tissue culture microscope, Olympus, IX70 P140603 Polaris II |
spellingShingle |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Comment Treatment Time in days Emiliania huxleyi, cell biovolume Bottle, Niskin Inverted tissue culture microscope, Olympus, IX70 P140603 Polaris II Armstrong, Evelyn Law, Cliff S Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
topic_facet |
adaptation Emiliania huxleyi Laboratory experiment Ocean acidification ocean warming subantarctic Species, unique identification Species, unique identification Semantic URI Species, unique identification URI Type of study Comment Treatment Time in days Emiliania huxleyi, cell biovolume Bottle, Niskin Inverted tissue culture microscope, Olympus, IX70 P140603 Polaris II |
description |
On four occasions during the two year incubation of a subantarctic E. huxleyi culture (2015-11-20 to 2017-12-01), from Day 309 onwards, cell size of the cultures was measured at cross-over points, after 7 – 8 generations while still in exponential growth phase. Cells from Now (11°C and pH 8.1) and Future (14°C and pH 7.8) cultures, and also the crossover experiments - Future cells inoculated into Now medium and incubated under Now conditions (Future in Now), and Now cells incubated in Future medium under Future conditions (Now in Future) were fixed in 1 % glutaraldehyde. Subsamples were mounted in a nanoplankton chamber (Phytotech) and measured with a calibrated eye-piece graticule under an Olympus IX70 inverted microscope. As the fixation process caused coccoliths to separate, the cell size measurements were for the cell body only, which allows more accurate determinations of cell volume (Buitenhuis et al., 2008). Cell volume was calculated assuming that the cells were spherical. ... : The study was supported by Coasts and Oceans Centre (Strategic Science Investment Fund of the National Institute of Water and Atmospheric Research). ... |
format |
Dataset |
author |
Armstrong, Evelyn Law, Cliff S |
author_facet |
Armstrong, Evelyn Law, Cliff S |
author_sort |
Armstrong, Evelyn |
title |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
title_short |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
title_full |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
title_fullStr |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
title_full_unstemmed |
Cell volume of Emiliania huxleyi measured at cross-over points during laboratory experiments ... |
title_sort |
cell volume of emiliania huxleyi measured at cross-over points during laboratory experiments ... |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://dx.doi.org/10.1594/pangaea.959827 https://doi.pangaea.de/10.1594/PANGAEA.959827 |
long_lat |
ENVELOPE(156.767,156.767,-80.217,-80.217) |
geographic |
Olympus |
geographic_facet |
Olympus |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1594/pangaea.959857 https://dx.doi.org/10.1371/journal.pone.0284415 https://dx.doi.org/10.4319/lo.2008.53.3.1181 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.95982710.1594/pangaea.95985710.1371/journal.pone.028441510.4319/lo.2008.53.3.1181 |
_version_ |
1809933475991519232 |