Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109 ...

• Main Authors: Bodur, Yasemin V, Felden, Janine, Braeckman, Ulrike
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: AODC; Event label; Latitude of event; Longitude of event; Date/Time of event; Elevation of event; Method/Device of event; Sample number; Filter; Depth, top/min; Depth, bottom/max; DEPTH, sediment/rock; Counts; Number of cells; Standard deviation; Microbial abundance, cells; Multicorer with television; Bottom lander; Acridine Orange Direct Counting AODC; Estimated from counts according to Hobbie et al. 1977; PS109; Polarstern; FRontiers in Arctic marine Monitoring FRAM; Arctic; Greenland
• Online Access: https://dx.doi.org/10.1594/pangaea.959552; https://doi.pangaea.de/10.1594/PANGAEA.959552

Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany). ... : Single cell abundances were calculated with the following equation:single cell abundance (cell number ml-1 sediment-1) = average cell count * (filtration funnel area/grid area) * dilutionwherefiltration funnel area = 203077576 µm²grid area = 15625 µm²dilution=4830 µl ...