Seawater carbonate chemistry and cellular and molecular changes in hemolymph and extrapallial fluid in Mercenaria mercenaria ...

• Main Authors: Schwaner, Caroline, Farhat, Sarah, Haley, John, Espinosa, Emmanuelle Pales, Allam, Bassem
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Containers and aquaria 20-1000 L or < 1 m**2; Immunology/Self-protection; Laboratory experiment; Laboratory strains; Mercenaria mercenaria; Mollusca; Not applicable; Other studied parameter or process; Single species; Type of study; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Local Time; pH; Haemolymph, pH; Haemolymph, pH, standard error; Extrapallial fluid pH; Extrapallial fluid pH, standard error; Category; Fluorescence intensity; Fluorescence intensity, standard error; Phagocytosis; Phagocytosis, standard error; Mortality; Mortality, standard error; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.957785; https://doi.pangaea.de/10.1594/PANGAEA.957785

Seawater pH and carbonate saturation are predicted to decrease dramatically by the end of the century. This process, designated ocean acidification (OA), threatens economically and ecologically important marine calcifiers, including the northern quahog (Mercenaria mercenaria). While many studies have demonstrated the adverse impacts of OA on bivalves, much less is known about mechanisms of resilience and adaptive strategies. Here, we examined clam responses to OA by evaluating cellular (hemocyte activities) and molecular (high-throughput proteomics, RNASeq) changes in hemolymph and extrapallial fluid (EPF—the site of biomineralization located between the mantle and the shell) in M. mercenaria continuously exposed to acidified (pH 7.3; pCO2 2700 ppm) and normal conditions (pH 8.1; pCO2 600 ppm) for one year. The extracellular pH of EPF and hemolymph (7.5) was significantly higher than that of the external acidified seawater (7.3). Under OA conditions, granulocytes (a sub-population of hemocytes important for ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-20. ...