Seawater carbonate chemistry and nitrogen fixation by phosphorus-limited Trichodesmium ...

• Main Authors: Zhang, Futing, Wen, Zuozhu, Wang, Shanlin, Tang, Weiyi, Luo, Yawei, Kranz, Sven A, Hong, Haizheng, Shi, Dalin
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Cyanobacteria; Entire community; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Micro-nutrients; North Pacific; Not applicable; Open ocean; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum; Tropical; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Replicate; Photosynthetic carbon fixation rate per cell; Nitrogen fixation rate, per cell; Particulate organic carbon, per cell; Particulate organic nitrogen per cell; Particulate organic phosphorus per cell; Polyphosphate as femto-equivalents of the standard per cell; Reduced nicotinamide adenine dinucleotide; Nicotinamide adenine dinucleotide phosphate; Nicotinamide adenine dinucleotide phosphate/Reduced nicotinamide adenine dinucleotide ratio; Gene expression; Adenosine 5-Triphosphate, per cell; Glutamate per cell; Carbon, organic, particulate/Phosphorus, organic, particulate ratio; Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio; Photosynthetic carbon fixation rate; Nitrogen fixation rate; Lipids per cell; Ribonucleic acid per cell; Cell, length; Cell, length, standard deviation; Cell, width; Cell, width, standard deviation; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.957342; https://doi.pangaea.de/10.1594/PANGAEA.957342

Growth of the prominent nitrogen-fixing cyanobacterium Trichodesmium is often limited by phosphorus availability in the ocean. How nitrogen fixation by phosphorus-limited Trichodesmium may respond to ocean acidification remains poorly understood. Here, we use phosphate-limited chemostat experiments to show that acidification enhanced phosphorus demands and decreased phosphorus-specific nitrogen fixation rates in Trichodesmium. The increased phosphorus requirements were attributed primarily to elevated cellular polyphosphate contents, likely for maintaining cytosolic pH homeostasis in response to acidification. Alongside the accumulation of polyphosphate, decreased NADP(H):NAD(H) ratios and impaired chlorophyll synthesis and energy production were observed under acidified conditions. Consequently, the negative effects of acidification were amplified compared to those demonstrated previously under phosphorus sufficiency. Estimating the potential implications of this finding, using outputs from the Community ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-04-07. ...