Seawater carbonate chemistry and gene expression in Mytilus edulis ...

• Main Authors: Mincarelli, Luana Fiorella, Chapman, Emma C, Rotchell, Jeanette M, Turner, Alexander P, Wollenberg Valero, Katharina C
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Gene expression incl. proteomics; Laboratory experiment; Laboratory strains; Mollusca; Mytilus edulis; Not applicable; Organic toxins; Single species; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Figure; Treatment; Sex; Stage; Gene expression; Salinity; Salinity, standard deviation; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.956746; https://doi.pangaea.de/10.1594/PANGAEA.956746

Plastic pollution and changes in oceanic pH are both pressing environmental issues. Little emphasis, however, has been placed on the influence of sex and gametogenesis stage when investigating the effects of such stressors. Here, we examined histology and molecular biomarkers of blue mussels Mytilus edulis exposed for 7 days to a pH 7.7 scenario (− 0.4 units) in combination with environmentally relevant concentrations (0, 0.5 and 50 µg/L) of the endocrine disrupting plasticiser di-2-ethylhexyl phthalate (DEHP). Through a factorial design, we investigated the gametogenesis cycle and sex-related expression of genes involved in pH homeostasis, stress response and oestrogen receptor-like pathways after the exposure to the two environmental stressors. As expected, we found sex-related differences in the proportion of developing, mature and spawning gonads in histological sections. Male gonads also showed higher levels of the acid–base regulator CA2, but females had a higher expression of stress response-related ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2022) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-03-13. ...