Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity ...

• Main Authors: Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Antarctica; apoptosis; Caspase; Cnemidocarpa verrucosa sp. A; laboratory study; Potter Cove; Superoxide Dismutase; Tunicata; Event label; Sample code/label; Type of study; Date/time start, experiment; Date/time end, experiment; Species; Treatment; Temperature, water; Apoptotic activity, per protein; Sampling by diver; Spectrophotometer UV/Vis, Beckman Coulter, DU800; Calculated average/mean values; Background corrected; Antarc*; Antarctic; King George Island; Ocean acidification; Southern Ocean
• Online Access: https://dx.doi.org/10.1594/pangaea.956183; https://doi.pangaea.de/10.1594/PANGAEA.956183

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Superoxide dismutase (SOD) activity was measured in the ascidia, samples (approximately 70 mg of brachial basket) were homogenized in 20 mM Tris-HCl, 1 mM EDTA, pH 7.6, with a ratio 1:4 w/v. Homogenates were centrifuged at 14,000 x g for 3 min at 4°C and the supernatant was used to measure SOD activity at 20°C ...