Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity ...

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying o...

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Bibliographic Details
Main Authors: Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José
Format: Dataset
Language:English
Published: PANGAEA 2023
Subjects:
Antarctica
apoptosis
Caspase
Cnemidocarpa verrucosa sp. A
laboratory study
Potter Cove
Superoxide Dismutase
Tunicata
Event label
Sample code/label
Type of study
Date/time start, experiment
Date/time end, experiment
Species
Treatment
Temperature, water
Apoptotic activity, per protein
Sampling by diver
Spectrophotometer UV/Vis, Beckman Coulter, DU800
Calculated average/mean values
Background corrected
Antarc*
Antarctic
King George Island
Ocean acidification
Southern Ocean
Online Access:https://dx.doi.org/10.1594/pangaea.956183
https://doi.pangaea.de/10.1594/PANGAEA.956183
Description
Summary:Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Superoxide dismutase (SOD) activity was measured in the ascidia, samples (approximately 70 mg of brachial basket) were homogenized in 20 mM Tris-HCl, 1 mM EDTA, pH 7.6, with a ratio 1:4 w/v. Homogenates were centrifuged at 14,000 x g for 3 min at 4°C and the supernatant was used to measure SOD activity at 20°C ...

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