Seawater carbonate chemistry and cellular inorganic carbon fluxes in Trichodesmium ...

• Main Authors: Eichner, Meri, Thoms, Silke, Kranz, Sven A, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Bacteria; Bottles or small containers/Aquaria <20 L; Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Trichodesmium erythraeum; Type; Species; Treatment; Time point, descriptive; Carbon fixation rate, per chlorophyll a; Carbon fixation rate, standard deviation; Gross carbon uptake rate, per chlorophyll a; Carbon uptake rate, standard deviation; Bicarbonate uptake rate, per chlorophyll a; Bicarbonate uptake in chlorophyll, standard deviation; Carbon dioxide uptake rate, per chlorophyll a; Carbon dioxide uptake, per chlorophyll, standard deviation; Ratio; Ratio, standard deviation; Carbon dioxide efflux, per chlorophyll a; Carbon dioxide efflux, per chlorophyll a, standard deviation; Salinity; Temperature, water; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.956021; https://doi.pangaea.de/10.1594/PANGAEA.956021

To predict effects of climate change on phytoplankton, it is crucial to understand how their mechanisms for carbon acquisition respond to environmental conditions. Aiming to shed light on the responses of extra- and intracellular inorganic C (Ci) fluxes, the cyanobacterium Trichodesmium erythraeum IMS101 was grown with different nitrogen sources (N2 vs NO3 –) and pCO2 levels (380 vs 1400 µatm). Cellular Ci fluxes were assessed by combining membrane inlet mass spectrometry (MIMS), 13C fractionation measurements, and modelling. Aside from a significant decrease in Ci affinity at elevated pCO2 and changes in CO2 efflux with different N sources, extracellular Ci fluxes estimated by MIMS were largely unaffected by the treatments. 13C fractionation during biomass production, however, increased with pCO2, irrespective of the N source. Strong discrepancies were observed in CO2 leakage estimates obtained by MIMS and a 13C-based approach, which further increased under elevated pCO2. These offsets could be explained by ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2023-02-20. ...