Seawater carbonate chemistry and proton gradients across the coral calcifying cell layer ...

• Main Authors: Venn, Alexander A, Tambutté, Eric, Comeau, Steeve, Tambutté, Sylvie
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Cnidaria; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Laboratory strains; Not applicable; Other; Single species; Stylophora pistillata; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Experiment; Experiment/study setup; Comment; Treatment; Temperature, water; Salinity; pH; pH, standard deviation; pH, extracellular; pH, extracellular, standard deviation; pH, intracellular; pH, intracellular, standard deviation; pH, mesoglea; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Thermometer; Confocal Microscope, Leica, SP5; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.953109; https://doi.pangaea.de/10.1594/PANGAEA.953109

In corals, pH regulation of the extracellular calcifying medium (ECM) by the calcifying cell layer is a crucial step in the calcification process and is potentially important to influencing how corals respond to ocean acidification. Here, we analyzed the growing edge of the reef coral Stylophora pistillata to make the first characterization of the proton gradient across the coral calcifying epithelium. At seawater pH 8 we found that while the calcifying epithelium elevates pH in the ECM on its apical side above that of seawater, pH on its basal side in the mesoglea is markedly lower, highlighting that the calcifying cells are exposed to a microenvironment distinct from the external environment. Coral symbiont photosynthesis elevates pH in the mesoglea, but experimental ocean acidification and decreased seawater inorganic carbon concentration lead to large declines in mesoglea pH relative to the ECM, which is maintained relatively stable. Together, our results indicate that the coral calcifying epithelium is ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-23.The experiments were done in the lab on coral samples that have been held in culture in Monaco for the last 30 years. The data file contains pH data from 3 experiments. In experiment 1corals were kept at seawater pH 8. In experiment 2. Corals were exposed to seawater acidification. In experiment 3 corals were exposed to manipulated DIC concentrations. The data are pH measurements taken from 3 internal compartments of the coral Stylophora pistillata: the mesoglea, the calcifying cells and the ECM. ...