Seawater carbonate chemistry and sperm swimming performance and pHi in the New Zealand sea urchin Evechinus chloroticus ...

• Main Authors: Hudson, Michael E, Sewell, M A
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Evechinus chloroticus; Laboratory experiment; Reproduction; FOS Medical biotechnology; Single species; South Pacific; Temperate; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Sperm motility; Sperm motility, standard error; Curvilinear velocity; Curvilinear velocity, standard error; Average path velocity; Average path velocity, standard error; Straight line velocity; Straight line velocity, standard error; Linearity; Linearity, standard error; Wobble; Wobble, standard error; Progression; Progression, standard error; pH; pH, standard error; Temperature, water; Salinity; Salinity, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.952309; https://doi.pangaea.de/10.1594/PANGAEA.952309

In sea urchins, spermatozoa are stored in the gonads in hypercapnic conditions (pH<7.0). During spawning, sperm are diluted in seawater of pH>8.0, and there is an alkalinization of the sperm's internal pH (pHi) through the release of CO2 and H+. Previous research has shown that when pHi is above 7.2-7.3, the dynein ATPase flagellar motors are activated, and the sperm become motile. It has been hypothesised that ocean acidification (OA), which decreases the pH of seawater, may have a narcotic effect on sea urchin sperm by impairing the ability to regulate pHi, resulting in decreased motility and swimming speed. Here we use data collected from the same individuals to test the relationship between pHi and sperm motility/performance in the New Zealand sea urchin Evechinus chloroticus (Valenciennes) under near- (2100) and far-future (2150) atmospheric pCO2 conditions (RCP 8.5: pH 7.77, 7.51). Decreasing seawater pH significantly negatively impacted the proportion of motile sperm), and four of the six ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-15. ...