Seawater carbonate chemistry of experiment on acclimatory gene expression of primed clams enhances robustness to elevated pCO2 ...

• Main Authors: Gurr, Samuel J, Trigg, Shelly A, Vadopalas, Brent, Roberts, Steven B, Putnam, H M
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Gene expression incl. proteomics; Laboratory experiment; Mollusca; North Pacific; Panopea generosa; Single species; Temperate; Type; Species, unique identification; Experiment; Treatment; Replicates; Salinity; Salinity, standard deviation; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Calculated using seacarb after Nisumaa et al. 2010; Calculated using seacarb after Orr et al. 2018; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.952281; https://doi.pangaea.de/10.1594/PANGAEA.952281

Sublethal exposure to environmental challenges may enhance ability to cope with chronic or repeated change, a process known as priming. In a previous study, pre-exposure to seawater enriched with pCO2 improved growth and reduced antioxidant capacity of juvenile Pacific geoduck Panopea generosa clams, suggesting that transcriptional shifts may drive phenotypic modifications post-priming. To this end, juvenile clams were sampled and TagSeq gene expression data were analysed after (i) a 110-day acclimation under ambient (921 μatm, naïve) and moderately elevated pCO2 (2870 μatm, pre-exposed); then following (ii) a second 7-day exposure to three pCO2 treatments (ambient: 754 μatm; moderately elevated: 2750 μatm; severely elevated: 4940 μatm), a 7-day return to ambient pCO2 and a third 7-day exposure to two pCO2 treatments (ambient: 967 μatm; moderately elevated: 3030 μatm). Pre-exposed geoducks frontloaded genes for stress and apoptosis/innate immune response, homeostatic processes, protein degradation and ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-12-12. ...