Seawater carbonate chemistry and the decrease of H+ concentration in the phycosphere and thickness of the pH boundary layer of marine diatoms Coscinodiscus wailesii ...

• Main Authors: Liu, Fengjie, Gledhill, Martha, Tan, Qiaoguo, Zhu, Kechen, Zhang, Qiong, Salaün, Pascal, Tagliabue, Alessandro, Zhang, Yanjun, Weiss, Dominik J, Achterberg, Eric Pieter, Korchev, Yuri
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Acid-base regulation; Bottles or small containers/Aquaria <20 L; Chromista; Coscinodiscus wailesii; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Single species; Type; Species, unique identification; Figure; Treatment; Proton gradients; Thickness; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Hydrogen ion concentration; Carbonate system computation flag; pH; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.951332; https://doi.pangaea.de/10.1594/PANGAEA.951332

Surface ocean pH is declining due to anthropogenic atmospheric CO2 uptake with a global decline of ~0.3 possible by 2100. Extracellular pH influences a range of biological processes, including nutrient uptake, calcification and silicification. However, there are poor constraints on how pH levels in the extracellular microenvironment surrounding phytoplankton cells (the phycosphere) differ from bulk seawater. This adds uncertainty to biological impacts of environmental change. Furthermore, previous modelling work suggests that phycosphere pH of small cells is close to bulk seawater, and this has not been experimentally verified. Here we observe under 140 μmol photons/m**2/s the phycosphere pH of Chlamydomonas concordia (5 µm diameter), Emiliania huxleyi (5 µm), Coscinodiscus radiatus (50 µm) and C. wailesii (100 µm) are 0.11 ± 0.07, 0.20 ± 0.09, 0.41 ± 0.04 and 0.15 ± 0.20 (mean ± SD) higher than bulk seawater (pH 8.00), respectively. Thickness of the pH boundary layer of C. wailesii increases from 18 ± 4 to ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-11-25. ...