Microbe quantification during short term anaerobic incubation measurements (CH4+CO2) from samples of Lena Delta collected in 2018 ...

• Main Authors: Laurent, Mélissa, Fuchs, Matthias, Treat, Claire C, Liebner, Susanne, Runge, Alexandra
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Anaerobic incubation; CH4; CO2; Laboratory experiment; Lena Delta; methanogens; methanotrophs; Permafrost; qPCR; Event label; Sample code/label; Latitude of event; Longitude of event; Date/Time of event; Location of event; Country; Site; Vegetation type; Ecosystem; Sample position; Soil type; Sample type; State of permafrost; Incubation duration; Time, incubation; Incubation temperature; Replicates; Depth, top/min; Depth, bottom/max; DEPTH, sediment/rock; Experimental treatment; DNA concentration; Volume; Dilution factor; Methane oxygenase, pmoA gene, abundance; Methyl coenzyme M reductase, mcrA gene, abundance; Fixed volume cylinder 250 cm3; SIPRE Auger; SYBRGreen quantitative PCR qPCR assay using the KAPA SYBRFAST qPCR Master Mix Sigma-Aldrich, Germany on CFX96 real-time thermal cycler Bio-Rad Laboratories Inc., United States; RU-Land_2018_Lena; AWI Arctic Land Expedition; The role of non-growing season processes in the methane and nitrous oxide budgets in pristine northern ecosystems FluxWIN; Aldrich; Arctic; lena delta; permafrost
• Online Access: https://dx.doi.org/10.1594/pangaea.945680; https://doi.pangaea.de/10.1594/PANGAEA.945680

The data sets were made during the summer 2021, with samples collected from three cores, at two depths (active and permafrost layers). In total, six samples (3 replicates by samples) were incubated for 67 days at two temperatures (4°C and 20°C). Core sampling were performed during the joint Russian-German LENA 2018 expedition. This data set compiles microbial quantification (DNA extraction + qPCR) for methanotrophs and methanogenic archaea. Key genes coding for the enzyme methyl coenzyme-M reductase (mcrA) (Thauer 1998) and for the enzyme particulate methane monooxygenase (pmoA) (Theisen et Murrell 2005) were examined to identify methanogens and methanotrophs, respectively. Microbial quantification was monitored at 3 different times during the incubation. Samples were collected before the incubation, after 60 days of incubation and after glucose addition. Due to the pandemic, it has not been possible to perform microbial quantification for all the samples after the glucose treatment. DNA extractions were ... : #999: below detection limit ...