Seawater carbonate chemistry and gene expression stability of Hong Kong oyster (Crassostrea hongkongensis) ...

• Main Authors: Dang, Xin, Noor, Zohaib, He, Yuanqiu, Lim, Yong Kian, Zhang, Yang, Yu, Ziniu, Thiyagarajan, Vengatesen
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Laboratory experiment; Magallana hongkongensis; Mollusca; North Pacific; Single species; Tropical; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Gene name; Stability; pH; Temperature, water; Salinity; Alkalinity, total; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.945551; https://doi.pangaea.de/10.1594/PANGAEA.945551

The increase of CO2 by anthropogenic activities leads to a decrease of pH in the ocean surface due to ocean acidification (OA) process. Generally, OA not only reduces the rate of calcification in marine environments but also affects various physiological activities, especially in calcifiers, including edible oysters. Quantitative real-time PCR (qRT-PCR) is often used to detect gene expression in response to OA, which relies on the stability of internal control. However, the appropriate internal controls for OA experiments remain scarce especially in the marine calcifiers. Hence, this study developed internal controls for qRT-PCR assays using the Hong Kong oyster (Crassostrea hongkongensis) as a model to reveal gene expression profile during development under OA. In this study, 17 housekeeping genes were selected as the possible candidate of the internal controls. After a comprehensive interpretation from the multiple algorithms and software, GAPDH paired with RL23 is recommended for the normalization for ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-06-20.The species name used in the paper is Crassostrea hongkongensis. ...