Cuticle relative atomic weight composition of juvenile California spiny lobsters (Panulirus interruptus) in response to ocean acidification ...

• Main Authors: Lowder, Kaitlyn, deVries, Maya S, Hattingh, Ruan, Day, James M D, Andersson, Andreas J, Zerofski, Phillip, Taylor, Jennifer
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: California Current Ecosystem; crustacean; Laboratory experiment; Ocean acidification; Type of study; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Specimen identification; Status; Body region; Cuticle layer; Identification; Treatment; Treatment pH; pH, standard deviation; Treatment temperature; Carbon; Nitrogen; Oxygen; Sodium; Magnesium; Aluminium; Silicon; Phosphorus; Sulfur; Chlorine; Calcium; Device type; Quality control; Trap, baited; Scanning electron microscope SEM equipped with electron-dispersive x-ray spectroscopy EDX
• Online Access: https://dx.doi.org/10.1594/pangaea.945338; https://doi.pangaea.de/10.1594/PANGAEA.945338

We examined the response of multiple structures used for predator defense in the California spiny lobster, Panulirus interruptus, to a series of ocean acidification-like conditions. Lobsters were collected by modified commercial traps offshore La Jolla, CA (in the area around 32.8534193, -117.2687516) in October 2016 and held at ambient conditions (pH 7.97, 16.5°C) before exposure to stable or diurnally fluctuating reduced pH conditions established by bubbling CO2 and as measured using best practices (ambient pH/stable, 7.97, 16.5°C; reduced pH/stable 7.67, 16.6°C; reduced pH with low fluctuations, 7.67 ± 0.05, 16.4°C; reduced pH with high fluctuations, 7.67 ± 0.10, 16.4°C). After three months, we examined the relative atomic weight composition (weight %/atomic weight of element) of the carapace using a scanning electron microscope (SEM) equipped with electron-dispersive x-ray spectroscopy (EDX). Each cuticle sample was rinsed with deionized water and allowed to air dry. Samples were then freeze-fractured ...