Seawater carbonate chemistry and blood and endolymph acid-base parameters in control and OA-exposed rockfish ...

• Main Authors: Kwan, Garfield Tsz, Tresguerres, Martin
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Acid-base regulation; Animalia; Bottles or small containers/Aquaria <20 L; Chordata; Coast and continental shelf; Laboratory experiment; Nekton; North Pacific; Pelagos; Sebastes diploproa; Single species; Temperate; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Treatment; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard error; Carbon dioxide; Carbon dioxide, standard error; Hydrogen ion concentration; Hydrogen ion concentration, standard error; pH; pH, standard error; Bicarbonate; Bicarbonate ion, standard error; Carbonate ion; Carbonate ion, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Replicates; Proteins; Proteins, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Aragonite saturation state; Calcite saturation state; Spectrophotometric; Potentiometric titration; Calculated using CO2SYS; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.945127; https://doi.pangaea.de/10.1594/PANGAEA.945127

Over a decade ago, ocean acidification (OA) exposure was reported to induce otolith overgrowth in teleost fish. This phenomenon was subsequently confirmed in multiple species; however, the underlying physiological causes remain unknown. Here, we report that splitnose rockfish (Sebastes diploproa) exposed to 1600 μatm pCO2 (pH 7.5) were able to fully regulated the pH of both blood and endolymph (the fluid that surrounds the otolith within the inner ear). However, while blood was regulated around pH 7.80, the endolymph was regulated around pH 8.30. These different pH setpoints result in increased pCO2 diffusion into the endolymph, which in turn leads to proportional increases in endolymph [HCO3−] and [CO32−]. Endolymph pH regulation despite the increased pCO2 suggests enhanced H+ removal. However, a lack of differences in inner ear bulk and cell-specific Na+/K+-ATPase and vacuolar type H+-ATPase protein abundance localization pointed out to activation of preexisting ATPases, non-bicarbonate pH buffering, or ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-06-08. ...