Cell growth and Particulate Organic Carbon / Nitrogen content of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment ...

• Main Authors: Balaguer, Jenna, Thoms, Silke, Trimborn, Scarlett
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: carbon fixation; FeC ratio; growth; iron; manganese; photophysiology; Phytoplankton; Southern Ocean; Trace metal; Treatment; Phaeocystis antarctica; Carbon, organic, particulate; Nitrogen, organic, particulate; Experiment day; In situ pump; Coulter counter, Beckman Coulter, Multisizer 3; Element analyser Euro EA 3000; PS112; Polarstern; Coulter; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.1594/pangaea.944456; https://doi.pangaea.de/10.1594/PANGAEA.944456

Cell counts and POC/PON content of P.antartica for the 4 different treatments were determined at the beginning and at the end of the experiment. The cell density and size were determined using a Beckman MultisizerTM 3 Coulter Counter® with a 100 μm aperture. For the POC/PON, 250 mL of water were filtered onto pre-combusted glass-fiber filters (15h, 500 ̊C, GF/F, ~0.6 μm, 25 mm, Whatman, Wisconsin, USA) and stored at -20°C in pre-combusted glass petri dishes. Prior to analysis with an Euro Elemental Analyzer 3000 CHNS-O (HEKAtech GmbH), the filters were dried for > 12h at 60 °C and then acidified with 200 μL of 0.2N HCL to remove inorganic carbon and dried a second time. Contents of POC and PON were corrected for blank measurements and normalized to filtered volume and cell densities to yield cellular quotas. ...