Seawater carbonate chemistry and Cu accumulation and cellular toxicity in the Pacific Oyster Crassostrea gigas ...

• Main Authors: Cao, Ruiwen, Zhang, Tianyu, Li, Xiao, Zhao, Y, Wang, Qing, Yang, Dinglong, Qu, Yi, Liu, Hui, Zhao, Jianmin
• Format: Dataset
• Language: English
• Published: PANGAEA 2019
• Subjects: Animalia; Behaviour; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Crassostrea gigas; Inorganic toxins; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Other studied parameter or process; Respiration; Single species; Temperate; Type; Species, unique identification; Species, unique identification URI; Species, unique identification Semantic URI; Experiment day; Treatment; Copper; Copper, standard deviation; Replicates; Clearance rate; Clearance rate, standard deviation; Respiration rate, oxygen; Respiration rate, oxygen, standard deviation; Condition index; Condition index, standard deviation; Acetylcholinesterase activity, unit per protein mass; Acetylcholinesterase activity, standard deviation; Superoxide dismutase activity, unit per protein mass; Superoxide dismutase activity, standard deviation; Glutathione S-transferase activity, unit per protein mass; Glutathione S-transferase activity, standard deviation; Lipid peroxidation, per protein; Lipid peroxidation, standard deviation; Pyruvate kinase activity, per protein; Pyruvate kinase activity, standard deviation; Hexokinase activity, per protein mass; Hexokinase activity, standard deviation; Integrated biomarker response index; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.944162; https://doi.pangaea.de/10.1594/PANGAEA.944162

Ocean acidification (OA) has been found to increase the release of free Cu2+ in seawater. However, only a handful of studies have investigated the influence of OA on Cu accumulation and cellular toxicity in bivalve species. In this study, Pacific oysters, Crassostrea gigas, were exposed to 25 μg/L Cu2+ at three pH levels (8.1, 7.8 and 7.6) for 14 and 28 days. Physiological and histopathological parameters [(clearance rate (CR), respiration rate (RR), histopathological damage and condition index (CI)), oxidative stress and neurotoxicity biomarkers [superoxide dismutase (SOD) and glutathione transferase (GST) activities, lipid peroxidation (LPO) and acetylcholinesterase (AChE) activity], combined with glycolytic enzyme activities [pyruvate kinase (PK) and hexokinase (HK)] were investigated in C. gigas. The bioconcentration of Cu was increased in soft tissues of Cu-exposed oysters under OA. Our results suggest that both OA and Cu could lead to physiological disturbance, oxidative stress, cellular damage, ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-05-13. ...