Seawater carbonate chemistry and growth rate, C fixation rate, cellular Fe uptake rates and growth Fe use efficiency of Phaeodactylum tricornutum ...

• Main Authors: Ye, Youting, Sunda, William G, Hong, Haizheng, Shi, Dalin
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Micro-nutrients; Not applicable; Ochrophyta; Other metabolic rates; Other studied parameter or process; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Treatment partial pressure of carbon dioxide; Growth rate; Growth rate, standard deviation; Carbon fixation rate; Carbon fixation rate, standard deviation; Cell iron/carbon ratio; Cell iron/carbon ratio, standard deviation; Iron uptake rate; Iron uptake rate, standard deviation; Growth Iron use efficiency; Growth Iron use efficiency, standard deviation; Photosynthetic Iron use efficiency; Photosynthetic Iron use efficiency, standard deviation; PsbA protein; Photosynthetic protein, PsbA, standard deviation; PsaC protein; Photosynthetic protein, PsbC, standard deviation; PsbA protein/PsaC protein ratio; PsbA protein/PsaC protein ratio, standard deviation; mRNA copy numbers ratio; mRNA copy numbers ratio, standard deviation; Iron, dissolved, inorganic; Irradiance; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.941907; https://doi.pangaea.de/10.1594/PANGAEA.941907

Light affects iron (Fe) growth requirements in marine phytoplankton while CO2 can influence energy allocation and light sensitivity. Therefore, ongoing increases in seawater CO2 concentrations could impact the growth of Fe- and light-limited phytoplankton. In this study, Phaeodactylum tricornutum was used as a model diatom to examine the interactive effects of Fe, light, and CO2 on photosynthesis, growth, and protein expression in marine phytoplankton. Low concentration of biologically available inorganic iron (Fe) and low-light intensity decreased specific rates of carbon (C)-fixation and growth, and the two together had an even greater effect, indicating a co-limitation. Increased partial pressure of CO2 from its current value (400 μatm) to 750 μatm had no effect at growth sufficient levels of Fe and light, but increased C-fixation and growth rate under Fe or light limitation, and had an even greater effect in Fe and light co-limited cells. The results suggest that ongoing increases in CO2 may increase ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-3-1. ...