Protein synthesis rates derived from incorporation of non-radioactively labeled phenylalanine in Antarctic fish ...

• Main Authors: Krebs, Nina, Tebben, Jan, Pörtner, Hans-Otto, Lannig, Gisela, Lucassen, Magnus, Mark, Felix Christopher, Bock, Christian
• Format: Dataset
• Language: English
• Published: PANGAEA 2022
• Subjects: 13C-labeling; Antarctica; cold adaptation; Energy budget; physiology; Sample code/label; Sample code/label 2; Subsample ID; Time in hours; Pachycara brachycephalum, standard length; Pachycara brachycephalum, mass; Pachycara brachycephalum, liver, mass; Hepato-somatic index; Sample, wet mass; Proteins, dry mass; Phenylalanine, unlabelled, in cytosol, area; Phenylalanine, labelled, in cytosol, area; Phenylalanine, labelled, in cytosol; Phenylalanine, unlabelled, in protein, area; Phenylalanine, labelled, in protein, area; Proteins, synthesis rate, per day; Experiment; Tape measure; Scale; Calculated; Liquid chromatography-high resolution mass spectrometry LC-HRMS with Orbitrap; Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.1594/pangaea.940626; https://doi.pangaea.de/10.1594/PANGAEA.940626

Temperature dependent growth is an important indicator to understand the thermal tolerance of organisms and to project their vulnerability to future climate change. Direct measurements of temperature dependent weight gains, however, are experimentally challenging and time consuming in long-lived species. Here, we reassess methodology to quantify the in vivo protein synthesis rate from amino acids, as a key component of growth. We developed an analytical method that is both robust against analytical errors and does not require hazardous radioactive materials. We utilized the incorporation of isotopically 13C15N-labeled-phenylalanine into fish muscle followed by quantification by liquid chromatography mass spectrometry to calculate accurate net protein synthesis rates in muscle tissue of Antarctic fish, Pachycara brachycephalum, in vivo. Specifically, we injected 150 mM of 13C9H915N1 phenylalanine intraperitoneally and sampled muscle tissue in 1,5h steps between 0 and 6 hours after injection. We quantified ... : ** DO NOT USE ** please refer to the dataset given in the "Replaced by" reference ...