Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ...
Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification...
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ftdatacite:10.1594/pangaea.939436 2024-04-28T08:31:27+00:00 Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y 2021 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.939436 https://doi.pangaea.de/10.1594/PANGAEA.939436 en eng PANGAEA https://cran.r-project.org/web/packages/seacarb/index.html https://dx.doi.org/10.1073/pnas.2101378118 https://cran.r-project.org/web/packages/seacarb/index.html Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Animalia Bottles or small containers/Aquaria <20 L Coast and continental shelf Echinodermata Gene expression incl. proteomics Laboratory experiment North Atlantic Pelagos Single species Strongylocentrotus purpuratus Temperate Zooplankton Type Species Registration number of species Uniform resource locator/link to reference Treatment Replicate Time in days Gene expression pH Carbon, inorganic, dissolved Alkalinity, total Partial pressure of carbon dioxide water at sea surface temperature wet air Temperature, water Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 dataset Dataset 2021 ftdatacite https://doi.org/10.1594/pangaea.93943610.1073/pnas.2101378118 2024-04-02T11:36:31Z Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification process by providing an exit route for protons liberated by the formation of CaCO3. Using the sea urchin larva, we examined the otopetrin ortholog otop2l, which is exclusively expressed in the calcifying primary mesenchymal cells (PMCs) that generate the calcitic larval skeleton. otop2l expression is stimulated during skeletogenesis, and knockdown of otop2l impairs spicule formation. Intracellular pH measurements demonstrated Zn2+-sensitive H+ fluxes in PMCs that regulate intracellular pH in a Na+/HCO3−-independent manner, while Otop2l knockdown reduced membrane proton permeability. Furthermore, Otop2l displays unique features, including strong activation by high extracellular pH (>8.0) and check-valve–like ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-12-16. ... Dataset North Atlantic Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animalia Bottles or small containers/Aquaria <20 L Coast and continental shelf Echinodermata Gene expression incl. proteomics Laboratory experiment North Atlantic Pelagos Single species Strongylocentrotus purpuratus Temperate Zooplankton Type Species Registration number of species Uniform resource locator/link to reference Treatment Replicate Time in days Gene expression pH Carbon, inorganic, dissolved Alkalinity, total Partial pressure of carbon dioxide water at sea surface temperature wet air Temperature, water Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 |
spellingShingle |
Animalia Bottles or small containers/Aquaria <20 L Coast and continental shelf Echinodermata Gene expression incl. proteomics Laboratory experiment North Atlantic Pelagos Single species Strongylocentrotus purpuratus Temperate Zooplankton Type Species Registration number of species Uniform resource locator/link to reference Treatment Replicate Time in days Gene expression pH Carbon, inorganic, dissolved Alkalinity, total Partial pressure of carbon dioxide water at sea surface temperature wet air Temperature, water Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
topic_facet |
Animalia Bottles or small containers/Aquaria <20 L Coast and continental shelf Echinodermata Gene expression incl. proteomics Laboratory experiment North Atlantic Pelagos Single species Strongylocentrotus purpuratus Temperate Zooplankton Type Species Registration number of species Uniform resource locator/link to reference Treatment Replicate Time in days Gene expression pH Carbon, inorganic, dissolved Alkalinity, total Partial pressure of carbon dioxide water at sea surface temperature wet air Temperature, water Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 |
description |
Otopetrins comprise a family of proton-selective channels that are critically important for the mineralization of otoliths and statoconia in vertebrates but whose underlying cellular mechanisms remain largely unknown. Here, we demonstrate that otopetrins are critically involved in the calcification process by providing an exit route for protons liberated by the formation of CaCO3. Using the sea urchin larva, we examined the otopetrin ortholog otop2l, which is exclusively expressed in the calcifying primary mesenchymal cells (PMCs) that generate the calcitic larval skeleton. otop2l expression is stimulated during skeletogenesis, and knockdown of otop2l impairs spicule formation. Intracellular pH measurements demonstrated Zn2+-sensitive H+ fluxes in PMCs that regulate intracellular pH in a Na+/HCO3−-independent manner, while Otop2l knockdown reduced membrane proton permeability. Furthermore, Otop2l displays unique features, including strong activation by high extracellular pH (>8.0) and check-valve–like ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-12-16. ... |
format |
Dataset |
author |
Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y |
author_facet |
Chang, William Weijen Matt, Ann-Sophie Schewe, Marcus Musinszki, Marianne Grüssel, Sandra Brandenburg, Jonas Garfield, David Bleich, Markus Baukrowitz, Thomas Hu, Marian Y |
author_sort |
Chang, William Weijen |
title |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
title_short |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
title_full |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
title_fullStr |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
title_full_unstemmed |
Seawater carbonate chemistry and otop2l expression of sea urchins (Stongylocentrotus purpuratus) ... |
title_sort |
seawater carbonate chemistry and otop2l expression of sea urchins (stongylocentrotus purpuratus) ... |
publisher |
PANGAEA |
publishDate |
2021 |
url |
https://dx.doi.org/10.1594/pangaea.939436 https://doi.pangaea.de/10.1594/PANGAEA.939436 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_relation |
https://cran.r-project.org/web/packages/seacarb/index.html https://dx.doi.org/10.1073/pnas.2101378118 https://cran.r-project.org/web/packages/seacarb/index.html |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.1594/pangaea.93943610.1073/pnas.2101378118 |
_version_ |
1797588978089590784 |