Phytoplankton pigment concentrations measured by HPLC in the surface water of the Mackenzie Delta Region during 4 expeditions from spring to fall in 2019 ...

• Main Authors: Matsuoka, Atsushi, Juhls, Bennet, Bécu, Guislain, Oziel, Laurent, Leymarie, Edouard, Lizotte, Martine, Ferland, Joannie, Doxaran, David, Maury, Juliette, Béguin, Marine, Laberge-Carignan, Audrey, Guilmette, Caroline, Hilborn, Andrea, Tisserand, Lucas, Devred, Emmanuel, Bossé-Demers, Thomas, Mannino, Antonio, Babin, Marcel
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: biogeochemistry; Biooptics; Coastal waters; hydrographic data; Mackenzie; Event label; Cruise/expedition; LATITUDE; LONGITUDE; DATE/TIME; Station label; DEPTH, water; Chlorophyll a; Chlorophyll b; Chlorophyll c; alpha-Carotene + beta-Carotene; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; Alloxanthin; Diadinoxanthin; Diatoxanthin; Fucoxanthin; Peridinin; Zeaxanthin; Monovinyl chlorophyll a; Chlorophyllide a; Monovinyl chlorophyll b; Chlorophyll c2 + chlorophyll c1 + Mg-2,4-divinyl pheoporphyrin; Chlorophyll c3; Lutein; Neoxanthin; Violaxanthin; Pheophytin a, total; Pheophorbide a, total; Prasinoxanthin; Chlorophyll total; Carotenoid pigments, photoprotective; Carotenoid pigments, photosynthetic; Pigments, photosynthetic; Carotenoids, total; Pigments, total accessory; Pigments, total; Pigments, total diagnostic; Pigments, accessory/chlorophyll a ratio; Carotenoid pigments, photosynthetic/Carotenoids, total ratio; Carotenoid pigments, photoprotective/Carotenoids, total ratio; Chlorophyll, total/Carotenoids, total ratio; Carotenoid pigments, photoprotective/Pigments, total; Carotenoid pigments, photosynthetic/Pigments, total; Chlorophyll a/Pigments, total; Hooker; Mackenzie Delta
• Online Access: https://dx.doi.org/10.1594/pangaea.937585; https://doi.pangaea.de/10.1594/PANGAEA.937585

The determination of phytoplankton using high performance liquid chromatography (HPLC) is detailed in Hooker et al. (2005; doi:10.1364/AO.44.000553) and Ras et al. (2008; doi:10.5194/bg-5-353-2008). Briefly, the pigments of particles retained on GF/F (0.7 µm) filters were extracted at -20°C with 3 mL methanol (100%). The filter samples were then disrupted using a sonicator and clarified one hour later by vacuum filtration through GF/F filters. The extracts were rapidly analyzed within 24h by HPLC with a complete Agilent Technologies system (comprising LC Chemstation software, a degasser, a binary pump, a refrigerated autosampler, a column thermostat and a diode array detector). ... : Empty cells = NA ...