Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni ...

• Main Authors: Servetto, Natalia, de Aranzamendi, M C, Bettencourt, Raul, Held, Christoph, Abele, Doris, Movilla, Juancho, González, G, Bustos, D M, Sahade, Ricardo José
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Animalia; Antarctic; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Laboratory experiment; Malacobelemnon daytoni; Other metabolic rates; Polar; Single species; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Day of experiment; Sample ID; Catalase activity, unit per protein mass; Superoxide dismutase activity, unit per protein mass; Gene expression; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; pH; pH, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Dry air column-averaged mixing ratio of carbon dioxide; Dry air column-averaged mixing ratio of carbon dioxide, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide in seawater, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Antarc*; Ocean acidification; Southern Ocean
• Online Access: https://dx.doi.org/10.1594/pangaea.936683; https://doi.pangaea.de/10.1594/PANGAEA.936683

Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 +/- 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively).Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-10-05. ...